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    20 April 2021, Volume 19 Issue 2 Previous Issue    Next Issue
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    New improvement is needed in diagnosis and treatment of tuberculosis among AIDS patients 
    SUN Jian-jun, LIU Li, LU Hong-zhou
    2021, 19 (2):  61-63. 
    Abstract ( 577 )   PDF (333KB) ( 1165 )  
    Both AIDS and tuberculosis are infectious diseases that seriously affect human health. At present,many difficulties exist in the diagnosis and treatment of tuberculosis in patients with AIDS. In order to further promote the prevention and treatment of tuberculosis among AIDS victims,the professional committee of AIDS combined with Tuberculosis of China Association for the Prevention and Treatment of STD / AIDS was established in Shanghai on November 28,2020 with the solid support of China Association for the Prevention and Treatment of STD / AIDS. This comment briefly introduces the current situation of AIDS complicated with tuberculosis in China and abroad,analyzes the real-word difficulties in the diagnosis and treatment of tuberculosis in AIDS patients,and prospects the future work and goals of the committee of AIDS complicated with Tuberculosis,so as to raise the continuous progress of the diagnosis and treatment of AIDS co-infected with tuberculosis in China.
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    Construction of prokaryotic expression system of HSP16-1 of Dermatophagoides farinae and functional identification of temperature stress response 
    NIU Dong-ling, ZHAO Ya-e, ZHANG Wan-yu, GUO Hong-song, HU Li
    2021, 19 (2):  64-69,81. 
    Abstract ( 687 )   PDF (3069KB) ( 805 )  
    Objective To establish a prokaryotic expression system and confirm the temperature stress response function of HSP16-1 of Dermatophagoides farinaeat protein level. Methods Based on the HSP16-1 gene sequence of D. farinae obtained by previous RNA-seq,specific primers were designed,amplified by PCR,cloned and sequenced. Bioinformatics software was used to analyze the physical and chemical properties,subcellular location and three-dimensional structure. The prokaryotic expression of plasmid pET32a / HSP16-1 was constructed and transformed into E. coli BL21. The expression of HSP16-1 recombinant protein was induced by 1 mmol / L IPTG at 16 ℃ ,28 ℃ and 37 ℃ ,and the samples were collected for SDS-PAGE analysis at 2 h, 4 h, 6 h and 8 h, respectively. The bacteria growth curves of the recombinant strains (pET32a / HSP16-1) and control strains (pET32a) under heat and cold stress were drawn. Results The sequencing results showed that the complete coding sequence ( CDS) of D. farinae HSP16-1 was 462 bp, which encoded 153 amino acids. BLAST alignment demonstrated that the nucleotide and amino acid sequences of D. farinae HSP16-1 were in similarity by 84. 63% and 87. 58%,respectively,with the closely related species D. pteronyssinus . Subcell were localized in the nucleus,and conservative region prediction revealed conservative region of α crystallin HSP23 superfamily. The recombinant plasmid pET32a / HSP16-1 was successfully constructed and identified by bacteria liquid PCR and double restriction enzyme digestion. SDS-PAGE analysis showed that HSP16-1 protein was successfully expressed,and the best induction condition was 37 ℃ for 6 h. The bacterial growth curve indicated that the growth of pET32a / HSP16-1 recombinant strains was better than that of pET32a control strains under heat stress,yet the growth of control strains was better than that of recombinant strains under cold stress. Conclusion HSP16-1 protein of D. farinae only generate stress response function under heat stress,yet does not show similar function under cold stress.

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    Prevalence of TORCH infection in hospitalized children with hematological diseases in a cancer hospital 
    ZHANG Qian, CHEN Shao-hua, SUN Yong-mei, CHEN Zhao-wu, LI Ming
    2021, 19 (2):  70-73. 
    Abstract ( 585 )   PDF (656KB) ( 1019 )  
    Objective To investigate the status of TORCH infection in children with hematological diseases in the First Affiliated Hospital of USTC (Western District),Anhui Provincial Cancer Hospital. Methods 354 children treated in the Department of Pediatric Hematology of our hospital were included from November 2015 to July 2019. Blood samples were obtained from all children, and detected for the TORCH-IgG and IgM using chemiluminescence immunoassay. Then the TORCH infection pattern, age distribution and disease types were analyzed in the 354 patients. Results TORCH test showed that the positive rate was 0. 6%,4. 2% and 7. 3% for Toxoplasma-immunoglobulin M (TOX-IgM),rubella virus-IgM (RV-IgM) and cytomegalovirus-IgM (CMV-IgM),and 21. 2%,88. 1%,91. 2%,49. 2% and 15. 8% for TOX-IgG,RV-IgG, CMV-IgG,HSV-Ⅰ-IgG and HSV-Ⅱ-IgG,respectively. Co-infection of CMV+RV occurred in one child. The infection rate of RV-IgM( +),CMV-IgM( +), RV-IgG( +),HSV-Ⅰ-IgG( +) or HSV-Ⅱ-IgG( +) was significantly different in different age group. Chi square trend test showed that the positive rates of RV-IgM,CMV-IgM and HSV-Ⅱ-IgG decreased with the age of children,while the positive rates of RV-IgG and HSV-Ⅰ-IgG increased with the age of children. The positive rate of TOXIgG,HSV-Ⅰ-IgG and HSV-Ⅱ-IgG was significantly different in children with diverse type of infection. Conclusion TORCH-IgG and IgM detection in children with hematological diseases can identify the type of infection and previous infection,and provide help for the diagnosis and treatment of hematological diseases.
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    Investigation on the parasitic infection of some marketed freshwater products in Fujian Province from 2014 to 2019
    CAI Wu-wei, LIN Chen-xin, JIANG Dian-wei, XIE Han-guo
    2021, 19 (2):  74-76,92. 
    Abstract ( 716 )   PDF (476KB) ( 1001 )  
    Objective To understand the parasitic infection status marketed freshwater products in Fujian province for evidences to develop scientific prevention and control strategies for the foodborne infection. Methods By the Monitoring Program for Food Contamination and Hazardous factors in Fujian province issued by Fujian provincial Health Commission,we conducted an investigation on the freshwater fish and snails sold in the supermarkets or farmer’s markets in the East,South, West,North and Central Fujian areas using stratified random sampling method. The samples were obtained and undergone detection of the larvae and metacercariae of the parasites via digestion technique,slide glass and autopsies. Results Totally,2 232 freshwater fish belonging to 26 species were collected from 2014 to 2019. The detection rate was 0. 09%(2 / 2 232) for Clonorchis sinensis ,3. 76% ( 84 / 2 232) for the larvae of Gnathostoma ,and 0. 17% ( 2 / 1 190) for the larvae of Angiostrongylus cantonensis in loach. No parasites were detected in the 90 samples of Cipangopaludina chinensis and 120 aliquots of Bellamya aeruginosa. Conclusion Food-borne parasitic infection exists to a certain degree in the fresh aquatic products sold in Fujiang areas,which suggests that intake of raw or under-cooked freshwater products can be great risks with parasitic infection. Therefore,health education should be strengthened and effective measures should be taken in order to ensure food safety in population.
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    Investigation on endemic situation of schistosomiasis in high-risk areas of Anhui Province during flood damage in 2020
    SUN Cheng-song, ZHU Hai, ZHANG Le-sheng, CAI Jun, GONG Yu-liang, XIE Wei-ping, ZHANG Shi-qing, WANG Tian-ping
    2021, 19 (2):  77-81. 
    Abstract ( 603 )   PDF (406KB) ( 1599 )  
    Objective To understand the epidemic situation of schistosomiasis in high-risk areas of Anhui Province during the flood damage in 2020 for taking effective measures to quickly respond to and eliminate the risk of infection. Methods Four high-risk environments of schistosomiasis associated with flood damage were selected in Chizhou,Tongling and Ma’anshan,the areas locating along the upper,middle and lower reaches of the Yangtze River within Anhui Province. Infectivity of the water body was detected using intelligent sentinel mice and bionic animal skin devices(About 20 sentinel mice and 40 bionic animal skin devices were set in each environment). In addition,demographic characteristics as well as the history of infection and contact with the epidemic water were obtained from the permanent residents and floating population aged 6 years and above in high-risk environments. Indirect hemagglutination assay kit was used to detect the antibodies against schistosome,and the results were subjected to epidemiological analysis. Results The intelligent sentinel mice retrieved from the water body within the four high-risk environments showed no infection with schistosome. Of the 40 pieces of bionic animal skin devices set in each water environment,specific DNA of cercariae was detected in 6 and 2,respectively retrieved  from Shengli town of Dongzhi County and Huyang town of Dangtu County. A total of 1 580 people around the high-risk environment underwent schistosoma antibody test,positive antibody was found 115,with an average positive rate of 7. 28%. The highest positive rate of blood test was seen in population in Huyang town of Dangtu County (10. 78%). There was significant difference in the positive rate of blood test between different genders ( χ2 = 17. 199,P<0. 001),yet the difference was insignificant between different age group and occupations. The positive rate of blood test was significantly higher in population with history of infection than in those without infection history( χ2 = 13. 144,P<0. 001),nevertheless,there was no statistical significance in the positive rate of blood test among population with or without history of exposure to the high-risk water environment,different reasons in contacting the infectious water and protective measures taken during contact in the past three months. Conclusion Transmission can be potential in the high-risk areas of schistosomiasis in the Yangtze River Basin within Anhui Province. In order to eliminate the risk and prevent resurgence of the epidemic,prevention and control measures against the risk should be put in place during and after the flood damage.
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    Survey of Sparganum mansoni infection in Xiangxi Autonomous Prefecture from 2010 to 2018 
    ZHANG Kai-ren, WANG Rui, GU Guan-jun, GONG Xi-wen, QÜ Bin-yan
    2021, 19 (2):  82-85. 
    Abstract ( 497 )   PDF (1282KB) ( 1044 )  
    Objective To understand the infection status of Sparganum mansoni in human and animal hosts in Xiangxi Autonomous Prefecture. Methods To collect and analyze the clinical cases and treatment records of patients with Sparganum mansoni  IgG antibody detection in parasite ward of Xiangxi Autonomous Prefecture CDC from 2010 to 2018,and epidemiological survey was conducted on the intermediate host and the host. Results Between 2010 and 2018,a total of 677 people (348 males and 329 females) underwent test of Sparganum mansoni infection. The results showed that 36 males and 20 female were positive for the antibody against Sparganum mansoni ,and the gender ratio was different( χ2 = 4. 06,P<0. 05). The oldest patient was 84 years old and the youngest one was 6 years old,and patients aged over 40 years accounted for 73. 21%. The difference was insignificant in different age group,ethnic group and occupations(P>0. 05). The most common infection was associated with intake of raw frogs and tadpoles,which accounted for 87. 50%. Forty-two pieces of Sparganum mansoni were detected in the frog muscles of 303 frogs dissected,with an infection rate of 13. 86%. Field microscopic examination of cat and canine feces revealed that the infection rate of Spirometra mansoni was 21. 74% and 5. 49%,respectively. Fifty-six patients were treated with surgery and praziquantel,albendazole and other drugs. Except for some brain types,other clinical types were cured. No obvious side effects occurred. Conclusion Spargnosis has become a common food-borne parasitic disease in Xiangxi Autonomous Prefecture. Infection with Spirometra Spagranum mansoni is prevalent in humans,frogs,
    cats and dogs,and human infection is associated with poor food habit in local population.

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    Investigation and analysis of paragonimiasis prevalence in Luoyang City from 2003 to 2019 
    LI Yun-xia, SUN Yi-Ying, LIANG Na, AI-Lin, TIAN Li-Guang
    2021, 19 (2):  86-88. 
    Abstract ( 477 )   PDF (646KB) ( 882 )  
    Objective To understand the incidence and epidemic characteristics of paragonimiasis in Luoyang area for evidence in prevention and control of this prevalence. Methods The clinical data were collected from the records of patients with paragonimiasis diagnosed in local Renhe Outpatient Clinic( a clinic for fever of unknown origin) and records maintained in the Information System for National Health Insurance and Disease Control of Luoyang City from 2003 to 2019,and then statistically analyzed pertinent to the distribution of endemic foci,time of treatment or onset,clinical characteristics and demographic characteristics. Results Totally,61 cases(37 males and 24 females) of paragonimiasis were annually reported in Luoyang from 2003 to 2019. The incidence was the most in 2011,with 16 cases reported. Luanchuan County had the largest number of cases(15 cases),followed Luoning County(13 cases),Songxian County(10 cases) and Yiyang County(6 cases),which accounted for 72. 13% of the total cases,and children under 10 years were most affected(55. 74%). 95. 08% of the patients had history of intake of uncooked or half-cooked creek crabs,drink of raw steam water or exposure to creek crabs before infection. Fever was seen in 63. 93% of the patients in the first clinic visit,and other symptoms were associated with cough,expectoration,mass at the abdominal and axillary region and others. Conclusion Prevalence of paragonimiasis is sporadic in population in Luoyang City. Nevertheless,it is necessary to strengthen the publicity and education in key population and key areas,as well as improve the diagnosis and treatment level and awareness of professionals in local institutions.
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    Analysis on the cause of death of patients with advanced schistosomiasis in Jinshan District of Shanghai City 
    YU Rui-fang, ZHU Yong-jia, SONG Can-lei, ZHU Jian-ming, ZHAO Yi-ming
    2021, 19 (2):  89-92. 
    Abstract ( 548 )   PDF (705KB) ( 1471 )  
    Objective To understand the basic information of death of patients with advanced schistosomiasis in Jinshan district,and analyze the causes of death for evidences to scientifically plan dynamic management strategies and specific treatment program for such patients. Methods The information was retrieved through follow-up of database of patients with advanced schistosomiasis from 2007 to 2019 in Jinshan district. The causes of death were analyzed pertinent to the death trend in patients with advanced schistosomiasis as well as the registered advanced cases in history and newly diagnosed advanced cases across the district. Results Death of advanced schistosomiasis patients was increased in fluctuant fashion in Jinshan district from 2007 to 2019. In early 2007,registered advanced cases were 1 121,and newly diagnosed advanced patients were 280 between 2007 and 2019. Totally,643 patients died of advanced schistosomiasis during 2007-2019,in whom 264 were males and 379 females. The age of the dead ranged from 50 to 97 years old,with a mean age of (76. 51±8. 45) years. The mortality was significantly higher in the newly diagnosed advanced patients than in those of registered in history ( χ2 = 53. 02,P<0. 01),and higher in females than in males( χ2 = 31. 39,P<0. 001). The top three causes of death in newly diagnosed advanced patients were associated with sequela and complications of schistosomiasis,liver cancer and cardiovascular and cerebrovascular diseases,which accounted for 57. 92%,16. 39% and 9. 29%,respectively. The top three causes for the death of historically registered advanced patients were involved in cardiovascular and cerebrovascular diseases,sequela and complications of schistosomiasis and other cancers,which accounted for 29. 78%,18. 04%,and 16. 52%,respectively. Conclusion Sequelae and complications of schistosomiasis,cardiovascular and cerebrovascular diseases,and liver cancer were the main causes responsible for the death of advanced schistosomiasis patients in Jinshan district during 2007- 2019. This suggests that treatment of such patients should be carried out according to the real condition by establishing a sound and effective care system for patients with advanced schistosomiasis.
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    Surveillance report on schistosomiasis in the key water area in Wuhan City from 2017 to 2019 using sentinel mice 
    ZHANG Jia-jing, LI Yang, WANG Hao, WANG Shuai, ZUO Yu-ting, XIONG Yue-lin, ZHOU Ye-hua, LUO Hua-tang, XU Ming-xing
    2021, 19 (2):  93-94,97. 
    Abstract ( 530 )   PDF (327KB) ( 1134 )  
    Objective To identify the high-risk environments of Schistosoma japonicum infection in the key water body in Wuhan area for reducing the risks of schistosomiasis. Methods The key water areas with most prevalence of Schistosoma japonicum in Yangtze River within the section of Wuhan City were selected,including Fuhe-Lunhe river system,Dongjing River-Tongshun River system and Jinshui River system. Sentinel mice were used to monitor the risks of Schistosoma japonicum infection. Results From 2017 to 2019,a total of 38 surveillance and forecast sites were detected in 21 administrative villages of 8 districts. Of the 760 sentinel mice placed,742 were recovered,with a recovery rate of 97. 63%. No positive infection and no adult worms were found in the 742 sentinel mice dissected. The infection rate of sentinel mouse was zero. Conclusion The infectivity of Schistosoma japonicum remains relative lower in the key water body in Wuhan area.
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    Analysis on the surveillance results of cholera in Jimei District of Xiamen City from 2011 to 2018
    QIU Yi-yan, OU Xiu-hua, CHEN Jia-xiang
    2021, 19 (2):  95-97. 
    Abstract ( 469 )   PDF (333KB) ( 944 )  
    Objective To understand the contamination status of Vibrio cholerae in aquatic products and external environment water body in Jimei District of Xiamen City through analyzing the monitoring results of cholera prevalence in Jimei District from 2011 to 2018 for early detection of this infection and evidence to plan effective prevention and control measures. Methods Samples were collected from aquatic products,external environment water and patients with diarrhea,and detected and analyzed in accordance with the procedures and standards specified in the National Cholera Surveillance Program, Diagnostic Criteria for Cholera(WS 289-2008) and Manual for Cholera Prevention and Control(6th Edition). Results Of the totally 4 509 samples detected in Jimei District from 2011 to 2018,six were positive( 0. 13%). The positive rate was 0. 19% and 0. 14%,respectively for aquatic products and external environment water. No Vibrio cholerae was detected in stool samples of diarrhea patients. In the 6 positive samples,4 were Vibrio choler O1 serotype Inaba,and 2 were O1 serotype Ogawa. No O139 Vibrio cholerae was detected. All strains were non-toxin producing. Conclusion O1 Vibrio cholerae occurs in the aquatic products and external environment water body in Jimei District,suggesting that surveillance of this infection should be strengthened. In addition,monitoring should be weighed in frogs and their aquaculture pond water besides well surveillance over the intestinal outpatient in order to timely discover pathogenic bacteria and infectious sources for corresponding prevention and control measures.
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    Severe fever with thrombocytopenia syndrome:Clinical comprehensive nursing in 35 cases
    KUAI Hui-fen, HUANG Bi-ling, QUAN Bin, HOU Wei-shun, ZHANG Qiang
    2021, 19 (2):  98-100. 
    Abstract ( 538 )   PDF (366KB) ( 1011 )  
    The clinical data of 35 hospitalized patients of severe fever with thrombocytopenia syndrome (SFTS) were retrospectively analyzed,and the clinical nursing work was summarized in this paper. SFTS is a condition characterized by acute onset and rapid progression,and requires early diagnosis and treatment as well as effective comprehensive nursing measures. In addition,appropriate isolation of the suspected or severe cases,protection of the nurses and physicians themselves in the ward,close observation on the changes of the patient’s condition,attention to the patient’s mental health,strengthened care
    of the patient’s daily life,and management and guidance after discharge are of great significance to prevent the virus transmission and improve the prognosis of such patients.

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    Molecular identification of Blattisocius keegani based on 18S rDNA gene sequences 
    LI Meng-zhu, ZHANG Lan-xiang, ZHAN Yu-juan, CHU Ling-miao, HU Ting-ting, LI Xin-mei, WANG Yan, SUN En-tao
    2021, 19 (2):  101-103,111. 
    Abstract ( 618 )   PDF (1445KB) ( 1281 )  
    Objective To establish a molecular method for identification of Blattisocius keegani based on ribosomal 18S rDNA gene sequences. Methods Storage samples were collected and isolated. Genomic DNA of a single mite was extracted on morphological identification basis. The COⅠand 18S rDNA gene sequences were obtained by PCR amplification,cloning and sequencing. Then the obtained sequences were aligned using Blast software,and 18S rDNA sequences of Blattisocius were retrieved from the GenBank. Multiple sequence alignment was done using the software Clustal(version 1. 83) with the known gene sequences. MEGA X software was used to analyze sequences,and the phylogenetic tree were constructed by neighbor-joining(NJ) method. Results The mites were identified as Blattisocius keegani based on COⅠ and morphological identification. The amplified sequences of the ten Blattisocius keegani were identical,showing A / T bias. The sequence homology of Blattisocius keegani with Blattisocius tarsalis and Blattisocius everti was 98. 73% and 98. 94%,respectively. Phylogenetic analysis based on 18S rDNA genes showed that the three mites were clustered into one branch. Conclusion We have successfully established a method for identifying Blattisocius keegani  on 18S rDNA sequences basis,which can be a foundation for accurate identification of Blattisocius keegani .
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    Observation on the effects of Muscovy duck preying on Bulinus globosus in Zanzibar 
    TANG Kai, CHEN Xiao-jun, HE Jian, WU Ping-jin, Saleh Juma Mohamed, WU Hong-chu, YANG Kun
    2021, 19 (2):  104-106. 
    Abstract ( 588 )   PDF (1164KB) ( 834 )  
    Objective To preliminarily investigate the effect of ecological molluscicidal technique by Muscovy duck preying on Bulinus globosus in schistosomiasis endemic area of Zanzibar in East Africa,part of Tanzania. Methods The research was implemented at the vegetable garden of the experimental base,a cooperation project for schistosomiasis control and prevention assisted by China. Two Muscovy ducks were fed in experimental area,and the control area was fenced to prevent entry of Muscovy ducks. By the 10th,20th,30th,and 40th day,the number of adult Bulinus globosus and their off-springs were comprehensively measured,respectively,and the survival rate of adult snails was calculated. The behavior of Muscovy ducks preying on Bulinus globosus was directly observed. Results The survival rate of Bulinus globosus was lower in the experimental area than in the control area by the 10th,20th,30th and 40th day ( χ2 = 7. 566,20. 671,18. 039,19. 200,respectively, P< 0. 05). The number of offspring snails in the experimental area was from 13 to 60 heads,which was lower than that in the control area(320~ 963 heads). Adult Bulinus globosus can be crushed by the beak or directly swallowed by the Muscovy ducks upon water filtering at the beak or swallowing vegetables,and the offspring snails were more easily swallowed because of their small size. Conclusion Ecological molluscicidal technique can be effective,because Muscovy duck may prey on Bulinus globosus,leading to inhibition on the reproduction of this snail.
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    Research progress in molecular biology diagnosis of toxoplasmosis 
    LIU Dao-hua, WANG Tian-ping
    2021, 19 (2):  107-111. 
    Abstract ( 604 )   PDF (420KB) ( 1692 )  
    Toxoplasmosis is a worldwide zoonotic parasitic infection caused by Toxoplasma gondii ,seriously endangering human health and animal husbandry production. Molecular biological techniques can provide a rapid,sensitive and specific detection for the diagnosis of this disease. This paper reviews the research progress in hybridization,polymerase chain reaction (PCR) and derivation,loop-mediated isothermal amplification (LAMP),gene chip and gene sequencing technology in the diagnosis of Toxoplasma gondii.
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    Difference of pathology induced by  Schistosoma japonicum and Schistosoma mansoni 
    CHEN Shu-xin, QIN Ming, ZHOU Fang-bin, HE Xing
    2021, 19 (2):  112-115,封三. 
    Abstract ( 1047 )   PDF (412KB) ( 1451 )  
    Schistosoma mansoni and Schistosoma japonica are two major pathogens of intestinal schistosomiasis worldwide. Primary pathology of the infection involved in these two parasites is designated by the egg-induced liver granuloma and fibrosis,yet the difference is significant in oviposition mode and constituent cells of the granuloma between the two infections. At resent, Schistosoma japonicum is the major study interest in China,whereas Schistosoma mansoni  is intensively investigated in foreign countries,and few reviews are available on the differences between the two kinds of schistosomiasis. In order to better understand the pathology diversity between Schistosoma japonicum and Schistosoma mansoni infection,this paper reviewed the pathological differences of the two parasites from six aspects,including the genome and evolutionary pathway of Schistosoma, larval migration,parasitic location and oviposition mode of adults,local histopathological findings,mechanisms of granulomatous formation and cell composition.
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    Considerations on implementation of ideological and political education in Human Parasitology for international students in China
    LONG Shao-rong, LIU Ruo-dan, ZHANG Xi, JIANG Peng, GAO Hua, ZHANG Jun-rong, CUI Jing, WANG Zhong-quan
    2021, 19 (2):  116-118. 
    Abstract ( 637 )   PDF (335KB) ( 851 )  
    This paper analyzed the necessity and current situation in carrying out the ideological and political education in the curriculum of Human Parasitology for international students in China,and proposed a new approach to introducing the ideological and political education into the curriculum in two aspects,including reform of teaching content and innovation of teaching methods,with an expect to provide a reference for the teaching of Human Parasitology for international students under current new situation.
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    Angiostrongyliasis:Report of one case with literature review 
    GU Mei-juan, LIU Xin-hua
    2021, 19 (2):  119-封三. 
    Abstract ( 506 )   PDF (332KB) ( 1196 )  
    This paper aims at investigating the clinical pictures of angiostrongyliasis,evidence for diagnosis and treatment measures as well as summary of the diagnosis and treatment experience in this infection through retrospective analysis of the clinical data in one case and literature review,with an attempt to improve the knowledge of clinicians in understanding of this disease in clinical practice.
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