Objective To clarify the influence on activity of AST, ALT, ALP and SDH for snails in project of afforestation for schistosomiasis control with different modes. Methods Three spots with different modes, cultivation with interval plantation, cultivation without plantation, no cultivation and plantation were selected in the project of afforestation for schistosomiasis control in Anhui Province, and three spots with grass land or reeds nearby were also selected. Snails in the spots selected were collected to assay the activity of AST, ALT, ALP and SDH. Results The activity of AST, ALT, and ALP for snails in woodland was obviously higher than that in grass land or reeds land, and SDH for snails was lower in woodland. The activity of AST、ALT、ALP and SDH for snails all was the highest in the mode of cultivation with interval plantation, the next was in the mode of cultivation without plantation, and the lowest was in the mode of no cultivation and plantation. Conclusion The change of ecological environment caused by project of afforestation for schistosomiasis control had certain influence on enzyme histochemistry of snails, which world disturb energetic metabolism of snails and affect the survival and reproduction on snails. The effect on snail control in the mode of cultivation with interval plantation would be better for its obviously influence on enzymatic activity of snails.