热带病与寄生虫学

热带病与寄生虫学 ›› 2025, Vol. 23 ›› Issue (1): 44-48.doi: 10.20199/j.issn.1672-2302.2025.01.009

• 实验研究 • 上一篇    下一篇

钉螺体内吸虫尾蚴的形态和分子特征鉴定

许月野1(), 许薇2(), 杨月阳3, 张安胜3, 杨志伟3, 王广辉3, 操治国2(), 赵金红1()   

  1. 1.皖南医学院医学寄生虫学教研室，安徽 芜湖 241002
    2.安徽省疾病预防控制中心
    3.皖南医学院临床医学院
  • 收稿日期:2024-05-30 出版日期:2025-02-20 发布日期:2025-04-03
  • 通信作者: 操治国,赵金红 E-mail:1792109763@qq.com;1606473833@qq.com;ahzhiguo@126.com;zhaojh@wnmc.edu.cn
  • 作者简介:许月野，女，硕士在读，研究方向：病原生物学。E-mail: 1792109763@qq.com；|许薇，女，硕士，卫生检验技师，研究方向：血吸虫病预防控制。E-mail: 1606473833@qq.com
  • 基金资助:
    安徽省大学生创新创业训练项目(S202110368064)

Morphological and molecular characteristics of cercaria in Oncomelania hupensis

XU Yueye1(), XU Wei2(), YANG Yueyang3, ZHANG Ansheng3, YANG Zhiwei3, WANG Guanghui3, CAO Zhiguo2(), ZHAO Jinhong1()   

  1. 1. Department of Medical Parasitology, Wannan Medical College, Wuhu 241002, Anhui Province, China
    2. Anhui Provincial Center for Disease Control and Prevention
    3. School of Clinical Medicine, Wannan Medical College
  • Received:2024-05-30 Online:2025-02-20 Published:2025-04-03
  • Contact: CAO Zhiguo,ZHAO Jinhong E-mail:1792109763@qq.com;1606473833@qq.com;ahzhiguo@126.com;zhaojh@wnmc.edu.cn

RichHTML

7

PDF (PC)

41

摘要：

目的 对安徽省室外钉螺饲养基地钉螺体内寄生的吸虫尾蚴进行形态和分子特征鉴定，了解其种属分类，为当地钉螺体内寄生吸虫的鉴定、防治提供分子生物学依据。方法 2023年4月底，采用系统抽样法采集安徽省室外饲养基地的钉螺，采用压碎镜检法观察钉螺体内尾蚴寄生情况，筛选阳性钉螺。提取尾蚴基因组DNA，采用PCR法扩增尾蚴内转录间隔区2（internal transcribed spacer 2, ITS2）并进行克隆测序，将测序结果在GenBank进行BLAST比对分析，通过构建系统发育树分析寄生吸虫尾蚴所属物种。结果 共检测300只钉螺，发现阳性钉螺12只。根据形态特征，寄生尾蚴分为无叉长尾形、无叉短尾形和双叉形3种，其对应的阳性钉螺分别为2只、1只和9只。基于ITS2基因的分子鉴定结果显示，无叉长尾形尾蚴与舌隐穴吸虫同源性为93.04%，为异形科隐穴吸虫属吸虫；无叉短尾形尾蚴与异囊坚盘吸虫同源性为98.02%，为枝腺科坚盘属吸虫；双叉形尾蚴与日本血吸虫同源性为99.62%~100.00%，为日本血吸虫。结论 除日本血吸虫外，安徽省钉螺还可能是舌隐穴吸虫与异囊坚盘吸虫的中间宿主。

关键词: 钉螺, 吸虫, 尾蚴, ITS2基因, 安徽省

Abstract:

Objective To identify the morphological and molecular characteristics of cercariae in the snail Oncomelania hupensis from outdoor feeding base in Anhui Province, and to understand their species classification for molecular biological basis in identification and control of the parasitic trematodes in local snails. Methods By systematic sampling, we obtained the snails at the end of April 2023 from an outdoor feeding base in Anhui Province. Then the samples were crushed to undergo microscopic examination of the presence of cercariae for screening positive snails. Genomic DNA was extracted from the cercariae, and the internal transcribed spacer 2 (ITS2) gene of cercaria was amplified using PCR, and then cloned and sequenced. The sequencing results were aligned with the genes in the GenBank using Basic Local Alignment Search Tool (BLAST). Finally, phylogenetic tree was constructed to analyze the species of parasitic fluke cercaria. Results A total of 300 snails were tested, with 12 testing positive. By the morphological characteristics, the parasitic cercariae fell into three forms, i.e., non-forked long-tailed, non-forked short-tailed and double-forked, which were present in 2, 1 and 9 of the positive snails, respectively. Molecular identification based on the ITS2 gene revealed that the homology of the non-forked long-tailed cercaria was 93.04% with Cryptocotyle lingua, belonging to the genus Cryptocotyle within the family Heterophyidae. The non-forked short-tailed cercaria showed 98.02% homology with Pycnoporus heteroporus and was classified as a trematode of the genus Pycnoporus within the family Lecithodendriidae, and the double-forked cercarial had a homology of 99.62%-100.00% with Schistosoma japonicum, which was Schistosoma japonicum. Conclusion In addition to Schistosoma japonicum, the Oncomelania hupensis in Anhui Province may also be intermediate hosts for Cryptocotyle sp. and Pycnoporus sp.

Key words: Oncomelania hupensis, Trematode, Cercaria, ITS2 gene, Anhui Province

中图分类号: