热带病与寄生虫学

热带病与寄生虫学 ›› 2013, Vol. 11 ›› Issue (2): 71-73,121.doi: 10.3969/j.issn.1672-2302.2013.02.003

• 论著 • 上一篇    下一篇

干扰素-γ、重组TGF-β1 RII蛋白对小鼠日本血吸虫病肝纤维化的影响

汪玉玲    叶芳    汪学龙*   

  1. 安徽医科大学病原生物学教研室（汪玉玲、汪学龙），滁州卫生学校（汪玉玲），中国科学院合肥物质科学研究院肿瘤医院（叶芳）， *通讯作者
  • 收稿日期:2013-03-01 出版日期:2013-02-25 发布日期:2013-06-08

Effects of IFN-γ and rTGF-β1 RII on hepatic fibrosis in mice infected with schistosomiasis japonica

Wang YuLing, Ye Fang, Wang Xuelong*.   

  1. Department of Parasitology, Anhui Medical University, Hefei 230032, China.
  • Received:2013-03-01 Online:2013-02-25 Published:2013-06-08

摘要： 目的  探讨IFN-γ、重组TGF-β1 RII蛋白对小鼠日本血吸虫病肝纤维化的影响。 方法  昆明小鼠（6～8周龄，体重18～25g，雌雄各半），随机分成6组（6只/组），正常对照组除外，其余30只通过腹部皮肤感染日本血吸虫尾蚴18±2条/鼠，六周后成功建立小鼠血吸虫性肝纤维化模型，具体治疗分组：①正常对照组；②模型对照组；③吡喹酮（PQT）治疗组；④PQZ +IFN-γ治疗组；⑤PQT+rTGF-β1RII蛋白治疗组；⑥PQT+IFN-γ+ rTGF-β1 RII蛋白治疗组。第九周末处死小鼠，HE染色法对肝组织进行病理学检查；TBA法测肝匀浆MDA含量。 结果  IFN-γ、rTGF-β1RII蛋白治疗显著降低肝纤维化指数，抑制日本血吸虫诱导的肝MDA水平的升高。 结论  日本血吸虫病化学药物治疗中，以PQT为基础配合rTGF-β1RⅡ蛋白、IFN-γ的联合疗法策略优于PQT单独用药策略；rTGF-β1RⅡ蛋白、IFN-γ的抗纤维化效应可能与其抗氧化功能有关。

关键词: 日本血吸虫病, γ-干扰素,  重组转化生长因子β1RⅡ型受体, 肝纤维化

Abstract:

Objective  To explore the effects of IFN-γ and rTGF-β1 RII on hepatic fibrosis in mice infected with schistosomiasis japonica.  Methods  36 Kunming mice (6-8 w,body weight 18-22g, male and female in half) were randomly divided into 6 groups (n=6), 6 mice were nomal control group, 30 mice were challenged percutaneously with 18±2 cercariae of Schistosoma japonicum and six weeks later they were randomly divided into 5 groups: model control group, Praziquantel (PQT) group, PQT+IFN-γ group, PQT + rTGF-β1RII group, and PQT+IFN-γ+rTGF-β1RII group. All the mice were killed at the end of the 9th week. The area of egg granuloma and degree of hepatic fibrosis were observed via HE and Masson stainings. The concentrations of liver homogenates MDA were detected by spectrophotometer using Assay Kits. Results  IFN-γ and /or TGF-β1RII protein significantly inhibited hepatic collagenous fibrosis in mice infected with schistosomiasis japonicum and reduced the concentrations of MDA that in liver tissue. Conclusion  The combination of IFN- γ and sTGF-β1RII with PQT had better effect on hepatic fibrosis in mice infected with schistosomiasis japonica than the single use of PQT. The anti-fibrosis effect of IFN-γ and sTGF-β1RII might relate with its antiantioxidant function.

Key words: Schistosomiasis japonica, IFN- γ, rTGF-β1RII , Hepatic fibrosis