热带病与寄生虫学

热带病与寄生虫学 ›› 2017, Vol. 15 ›› Issue (1): 31-35.doi: 10.3969/j.issn.1672-2302.2017.01.009

• 论著 • 上一篇    下一篇

日本血吸虫成虫的非编码RNA高通量测序分析

喻祎哲，杨杰，曾凡胜，王红梅，秦志强*   

  1. 413000 湖南益阳市，湖南省益阳市第一中学（喻祎哲），湖南省益阳医学高等专科学校（曾凡胜、杨杰、王红梅），中国疾病预防控制中心寄生虫病预防控制所（秦志强）；*通讯作者
  • 出版日期:2017-03-10 发布日期:2017-04-12
  • 基金资助:

    湖南省教育厅科研课题（项目编号：16C1598）

Analysis of the non-coding RNA in adult Schistosoma japonicum by high-throughput sequencing

Yu Yizhe1, Yang Jie2, Zeng Fansheng2, Wang Hongmei2, Qin Zhiqiang3*   

  1. 1. No. 1 Middle School of Yiyang City,  Yiyang 413000, China. 2. Yiyang Medical College,Yiyang 413000, China. 3. National Institute of Parasitic Diseases, Shanghai 200025, China. * Corresponding Author.
  • Online:2017-03-10 Published:2017-04-12

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摘要： 目的  通过对日本血吸虫成虫做高通量测序分析，鉴定已知miRNA的表达水平，预测新miRNA的序列结构及生物学功能。 方法  首先提取日本血吸虫成虫miRNA进行深度测序，其次对miRNA做数据分析、统计长度分布与比对注释，鉴定已知miRNA表达水平，最后对发现的新miRNA序列进行结构和功能的预测。 结果  将构建文库中日本血吸虫表达的miRNA与最新Sanger miRBase数据库中的miRNA比对。结果显示，在鉴定的miRNA中，sja-mir-125b是表达量最高的miRNA，另外sja-bantam，sja-mir-10，sja-mir-71a，sja-mir-36，sja-mir-61的表达量也较高，上述六种miRNA表达量占到已知miRNA表达量的94.6%。本研究发现了十种在日本血吸虫表达量最高的新miRNA，并对新miRNA进行序列及结构的预测。 结论  日本血吸虫成虫新miRNAs例如sja-mir-125b的发现将为深入研究其在血吸虫发育与致病的生物学功能打下基础。

关键词: 非编码核糖核酸, miRNA, 高通量测序, 日本血吸虫病, 成虫

Abstract:

Objective  To predict the miRNA sequence an its biological function by determining the miRNA expression level in adult Schistosoma japonicum with high-throughput sequencing technique. Methods The miRNAs of Schistosoma japonicum were extracted and analyzed by high-throughput sequencing. Length distribution and expression levels of miRNAs were identified and annotated. Then the structure and expression of newly identified miRNA sequences were predicted with software tool, and aligned with Schistosoma japonicum miRNAs in library of latest Sanger miRBase database. Results In the Schistosoma japonicum miRNAs, sja-mir-125b was maximally expressed, followed by sja-bantam, sja-mir-10, sja-mir-71a, sja-mir-36 and sja-mir-61, which accounted for 94.6% of the known miRNAs. Additionally, ten new miRNAs of Schistosoma japonicum were found, and their sequences and structures were further predicted. Conclusion Novel miRNAs, especially sja-mir-125b detected in our work may lay a foundation for following research in Schistosoma japonicum development and pathogenic.

Key words: Non-coding RNAs, microRNA, High-throughput sequencing, Schistosoma japonicum, Adult worm