热带病与寄生虫学

热带病与寄生虫学 ›› 2015, Vol. 13 ›› Issue (4): 189-192.doi: 10.3969/j.issn.1672-2302.2015.04.002

• 论著 • 上一篇    下一篇

基于可溶性童虫抗原（SSA）的IHA法早期诊断日本血吸虫病的初步研究

孙成松，章乐生，汪峰峰，王玥，尹晓梅，周莉，汪奇志，张世清，汪天平*   

  1. 230061 合肥市，安徽省血吸虫病防治研究所
  • 出版日期:2015-12-10 发布日期:2016-01-05
  • 基金资助:

    国家科技重大专项（2012ZX10004-220，2008ZX10004-011）；安徽省自然科学基金项目（1408085MH194）

Preliminary study on early diagnosis of schistosomiasis japonicum by IHA based on soluble schistosomula antigen (SSA)

Sun Chengsong, Zhang Lesheng, Wang Fengfeng, Wang Yue, Yin Xiaomei, Zhou Li, Wang Qizhi, Zhang Shiqing, Wang Tianping*   

  1. Anhui Provincial Institute of Schistosomiasis Control, Hefei 230061, China
  • Online:2015-12-10 Published:2016-01-05

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摘要： 目的  建立基于可溶性童虫抗原（SSA）早期诊断日本血吸虫病的IHA法。 方法  收集日本血吸虫感染后第13d童虫，制备SSA；探索SSA致敏红细胞的最佳体积比例以及灭活补体阴性兔血清的最佳封闭浓度，制备基于SSA的IHA法诊断试剂（以下简称“SSA-IHA法”），并检测日本血吸虫感染前及感染后第1、2、3、4、5、6周兔血清。 结果  SSA致敏红细胞的最佳体积比例为1:1，灭活补体阴性兔血清的最佳封闭浓度（V/V）为3%。SSA-IHA法检测日本血吸虫感染前及感染后第1、2、3、4、5、6周兔血清的阳性率依次为0%、0%、0%、90.91%、100%、100%和100%。 结论  基于可溶性童虫抗原（SSA）的IHA法在早期诊断日本血吸虫病方面具有一定的价值。

关键词: 可溶性童虫抗原, 间接红细胞凝集试验, 早期诊断, 日本血吸虫病

Abstract:

Objective  To develop an IHA method by using soluble schistosomula antigen (SSA) for early diagnosis of schistosomiasis japonica. Methods  Schistosomula were collected 13 days post-infection and SSA were prepared. The optimal volume ratio between SSA and erythrocyte and the best blocking concentration (V/V) by negative rabbit sera of inactivating complement were explored. Then the IHA method based on SSA (SSA-IHA) was constructed and the sera from non-infected rabbits and 1, 2, 3, 4, 5, 6 weeks post-infection were detected. Results  The optimal volume ratio between SSA and erythrocyte was 1∶1 and the best blocking concentration (V/V) by negative rabbit sera of inactivating complement was 3%. The positive rate of sera from non-infected rabbits and 1, 2, 3, 4, 5, 6 weeks post-infection were 0%, 0%, 0%, 90.91%, 100%, 100%, and 100%, respectively when tested by SSA-IHA. Conclusion  The IHA method based on SSA has value on early diagnosis of schistosomiasis japonica.

Key words: Soluble schistosomula antigen (SSA), Indirect hemagglutination assay (IHA), Early diagnosis, Schistosomiasis japonicum