热带病与寄生虫学

热带病与寄生虫学 ›› 2016, Vol. 14 ›› Issue (1): 25-28.doi: 10.3969/j.issn.1672-2302.2016.01.009

• 论著 • 上一篇    下一篇

四种单味中药及其复方对小鼠血吸虫病肝纤维化Ⅰ、Ⅲ型胶原及TGF-β1表达的影响

贺佩，胡君健，何永康，喻鑫玲，王媛园，罗振华，曹建平*   

  1. 414000 湖南岳阳市，湖南省血吸虫病防治所（贺佩、胡君健、何永康、喻鑫玲、王媛园、罗振华）， 国家卫生计生委寄生虫病原与媒介生物学重点实验室、中国疾病预防控制中心寄生虫病预防控制所（曹建平）
  • 出版日期:2016-03-10 发布日期:2016-04-11
  • 基金资助:

    卫生部寄生虫病原与媒介生物学重点实验室开放课题资助项目（WSBKTKT201303）；国家血吸虫病临床重点专科建设项目

Effects of single use and integrated use of four herbs on the expression of Type Ⅰ/Ⅲ collagen and TGF-β1 in schistosomiasis japonica mice with liver fibrosis

He Pei1, Hu Junjian1, He Yongkang1, Yu Xingling1, Wang Yuanyuan1, Cao Jianping2*   

  1. 1. Hunan Institute of Parasite Disease, Yueyang 414000, China;  2. Key Laboratory of Parasite and Vector Biology, Ministry of Health;  National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, China.
  • Online:2016-03-10 Published:2016-04-11

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摘要： 【摘要】 目的  研究当归、黄芪、丹参和赤芍四种单味中药及其复方对日本血吸虫病早期肝纤维化的小鼠肝组织Ⅰ型胶原α1(Ⅰ)链COL1A1、Ⅲ型胶原α1(Ⅲ)链COL3A1及转化生长因子β1 (TGF-β1) mRNA表达的影响。 方法  建立日本血吸虫病肝纤维化小鼠模型，随机分为6组：A组（当归组），B组（黄芪组），C组（丹参组），D组（赤芍组），E组（当归、黄芪、丹参、赤芍复方组）和F组（吡喹酮组），其中F组用吡喹酮单独治疗，其他各组先用吡喹酮治疗后再用中药治疗，同时设有N组对照，各组内按照治疗期长短又分为1组（20天组）和2组（40天组）。治疗结束后应用实时荧光定量PCR（Real-time PCR）的方法检测小鼠肝组织中COL1A1、COL3A1及TGF-β1 mRNA的表达。 结果  A、B、C、D、E组COL1A1、COL3A1及TGF-β1 mRNA的表达明显低于F组，P<0.001；E组的表达量最低，E1组的COL1A1、COL3A1及TGF-β1 mRNA的表达显著低于A1、B1、C1、D1组，P<0.05～0.001；E2组COL1A1表达明显低于A2、B2、D2，P<0.05，E2组COL3A1和TGF-β1的表达明显低于B2、D2组，P<0.05。 结论  单味中药当归、黄芪、丹参、赤芍及其复方均有抗血吸虫病肝纤维化的作用，能显著降低Ⅰ、Ⅲ型胶原的生成，抑制TGF-β1的表达，复方比单方能更好更快的发挥疗效。

关键词: 中药, 日本血吸虫病, 肝纤维化, 胶原, TGF-&beta, 1

Abstract:

【Abstract】 Objectives  To examine the effects by single use and integrated use of the four herbs Angelica sinensis, Astragalus mongholicus, Salvia miltiorrhiza, Radix Paeoniae Rubra on mRNA expression of type I collagen α1 (Ⅰ) chain (COL1A1)gene, type Ⅲ collagen α1(Ⅲ) chain(COL3A1)gene and TGF-β1 gene in liver tissues of hepatic fibrosis mice due to schistosoma japonicum infection. Methods  Liver fibrosis models from schistosomiasis were developed in mice and then randomized into group A(treated with Angelica sinensis), B(treated with Astragalus mongholicus), C(Salvia miltiorrhiza), D(Radix Paeoniae RubraⅡ), E(integrated treatment with the four herbs) and F(treated with praziquantel). Group F received simple praziquantel, and remaining groups were given additional herbal components besides praziquantel. Group N was used as controls, the six groups were subgrouped into two by the treatment course(20 days and 40 days). After treatment, Real-time PCR was performed to determine the mRNA of COL1A1, COL3A1 and TGF-β1. Results  Group A, B, C, D and E had better treatment effects on reduction of the mRNA expression of COL1A1, COL3A1 and TGF-β1 compared to group F group(P<0.001). Among treatment group, mRNA expression of COL1A1, COL3A1 and TGF-β1 in group E1 was significantly lower than that of group A1, B1, C1and D1(P<0.05 or P<0.001).Group E2 had lower mRNA expression of COL1A1 than group A2, B2 and D2(P<0.05), and expression of COL3A1 and TGF-β1 was lower than that of group B2 and D2(P<0.05). Conclusion  Either single use or integrated use of the four herbs Angelica sinensis, Astragalus mongholicus, Salvia miltiorrhiza, Radix Paeoniae Rubra can improve the liver fibrosis from schistosomiasis and significantly downregulate mRNA expression of COL1A1, COL3A1 and TGF-β1. However, integrated use of the four herbs may produce better and faster effects than single recipe．

Key words: Chinese herbal medicine, Schistosomiasis japonica, Liver fibrosis, Collagen, TGF-β1