热带病与寄生虫学

热带病与寄生虫学 ›› 2023, Vol. 21 ›› Issue (4): 210-215.doi: 10.3969/j.issn.1672-2302.2023.04.007

• 论著 • 上一篇    下一篇

我国弓形虫Chinese 1优势基因型感染对小鼠组织中微量金属元素代谢的影响

幸忆恩(), 蔡亦红()   

  1. 安徽医科大学公共卫生学院卫生检验与检疫学系，安徽医科大学病原生物学安徽省重点实验室，安徽医科大学人畜共患病安徽高校省级重点实验室，安徽 合肥 230032
  • 收稿日期:2023-01-17 出版日期:2023-08-20 发布日期:2023-08-23
  • 通信作者: 蔡亦红 E-mail:664106716@qq.com;yihongcai2022@163.com
  • 作者简介:幸忆恩，女，硕士在读，研究方向：感染与免疫。E-mail: 664106716@qq.com
  • 基金资助:
    安徽省高校自然科学研究重点项目(KJ2020A0153);安徽医科大学博士基金项目(XJ202005);安徽医科大学基础和临床合作研究计划项目(2021xkjT033)

Effects of Toxoplasma gondii Chinese 1 genotype strain infection on trace element metabolism in mice tissues

XING Yien(), CAI Yihong()   

  1. Department of Health Inspection and Quarantine, School of Public Health, Anhui Medical University, The Key Laboratory of Microbiology and Parasitology of Anhui Province, Anhui Medical University, The Provincial Key Laboratory of Zoonoses of High Institutions in Anhui, Anhui Medical University, Hefei 230032, Anhui Province, China
  • Received:2023-01-17 Online:2023-08-20 Published:2023-08-23
  • Contact: CAI Yihong E-mail:664106716@qq.com;yihongcai2022@163.com

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摘要：

目的 观察弓形虫Chinese 1优势基因型（ToxoDB#9）弱毒株TgCtwh6感染小鼠后，其体内微量金属元素的留存情况，探讨TgCtwh6感染对小鼠各组织金属元素代谢的影响。方法 建立TgCtwh6感染小鼠急性期模型（急性期组）和慢性期模型（慢性期组），同时设置对照组，实验小鼠6只/组。运用电感耦合等离子体质谱仪检测不同组别小鼠心脏、肝脏、脾脏和肾脏内的Fe2+、Cu2+、Mn2+、Zn2+、Mg2+等金属元素水平，Western blot检测不同组别小鼠肝脏内金属元素转运体ZIP 8（Zrt- and Irt-like protein 8）的蛋白表达，超氧化物歧化酶（superoxide dismutase，SOD）活性检测试剂盒检测SOD活性，对不同组别的检测结果进行统计分析。结果 相对于对照组，急性期组和慢性期组小鼠肝脏内Fe2+、Mn2+和Zn2+含量均上调[（76.348±11.613） μg/g和（88.545±10.555） μg/g vs. （52.388±6.165） μg/g；（1.298±0.065） μg/g和（1.405±0.066） μg/g vs. （1.141±0.110） μg/g；（17.506±0.824） μg/g和（17.031±0.907） μg/g vs. （15.650±0.305） μg/g]；脾脏内Fe2+、心脏内Cu2+和Zn2+含量均下调[（128.519±13.531） μg/g和（120.309±10.023） μg/g vs. （147.720±15.303） μg/g；（5.189±0.255） μg/g和（5.631±0.485） μg/g vs. （6.440±0.740） μg/g；（17.034±0.974） μg/g和（16.858±0.953） μg/g vs. （19.258±0.965） μg/g]。急性期组小鼠心脏内Mn2+和Mg2+含量均低于对照组[（0.605±0.052） μg/g vs. （0.686±0.051） μg/g；（153.346±3.221） μg/g vs. （163.012±8.204） μg/g]。与对照组相比，急性期组和慢性期组小鼠肝脏ZIP 8蛋白表达均上调，且慢性期组高于急性期组（F=35.240，P<0.05）。对照组、急性期组和慢性期组小鼠肝脏SOD活性分别为（14.041±0.734） U/mL、（16.463±0.616） U/mL和（15.859±0.780） U/mL，组间差异有统计学意义（F=9.370，P<0.05）。结论 TgCtwh6急性和慢性感染状态下小鼠不同组织中部分微量金属元素含量会发生上调或者下调，同时出现ZIP 8表达上调激活SOD活性，从而发挥肝脏保护作用。

关键词: 刚地弓形虫, 小鼠, Chinese 1基因型, 微量金属元素, 肝脏

Abstract:

Objective To measure the retention of trace elements in different tissues of the body in mice infected by weakly virulent strain TgCtwh6, the dominant genotype of Chinese 1 (ToxoDB #9), and investigate the effects of the infection on metabolism of trace metal elements in the mouse tissues. Methods Mouse models of TgCtwh6 at acute and chronic infection were established respectively, and control group was established (n=6 for each group). The levels of various metal elements (Fe2+, Cu2+, Mn2+, Zn2+, Mg2+ ) in different tissues (heart, liver, spleen and kidney) of the infected mice were determined by inductively coupled plasma-mass spectrometry (ICP-MS). Western blot was performed to measure the protein expression of the metal element transporter ZIP 8 (Zrt- and Irt- like protein 8), and superoxide dismutase (SOD) activity kits were used to detect the activity of SOD in liver tissues. Finally, the results obtained in each group were statistically analyzed. Results Compared to the control group, the levels of Fe2+, Mn2+ and Zn2+ in the liver of the mice at acute and chronic infection were increased [(76.348±11.613) μg/g and (88.545±10.555) μg/g vs. (52.388±6.165) μg/g, (1.298±0.065) μg/g and (1.405±0.066) μg/g vs. (1.141±0.110) μg/g, (17.506±0.824) μg/g and (17.031±0.907) μg/g vs. (15.650±0.305) μg/g, respectively], yet the content of Fe2+ in spleen, Cu2+ and Zn2+ in heart was downregulated [(128.519±13.531) μg/g and (120.309±10.023) μg/g vs. (147.720±15.303) μg/g, (5.189±0.255) μg/g and (5.631±0.485) μg/g vs. (6.440±0.740) μg/g, (17.034±0. 974) μg/g and (16.858±0.953) μg/g vs. (19.258±0.965) μg/g, respectively]. The levels of Mn2+ and Mg2+ in the heart of mice at acute infection were lower than those of control group [(0.605±0.052) μg/g vs. (0.686±0.051) μg/g, (153.346±3.221) μg/g vs. (163.012±8.204) μg/g, respectively]. Protein ZIP 8 was up-regulated in the liver of mice at acute and chronic infection as compared with that of control group, and the protein level was higher at chronic infection than at acute infection (F=35.240, P<0.05). SOD activity was (14.041±0.734) U/mL, (16.463±0.616) U/mL and (15.859±0.780) U/mL, respectively, in the liver of mice in the control group, mice at acute and chronic infection. The differene was significant (F=9.370, P<0.05). Conclusion Infection of the mice with TgCtwh6, either at acute or chronic infection, can lead to up-regulation or down-regulation of partial trace metal elements in the tissues of mice as well as upregulated ZIP 8 expression, which may activate SOD activity and function roles in protecting the liver.

Key words: Toxoplasma gondii, Mice, Chinese 1 genotype strain, Trace metal elements, Liver

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