热带病与寄生虫学

热带病与寄生虫学 ›› 2017, Vol. 15 ›› Issue (1): 19-21.doi: 10.3969/j.issn.1672-2302.2017.01.005

• 论著 • 上一篇    下一篇

小鼠感染日本血吸虫尾蚴时龄与粪便虫卵排出关系的研究

孙成松，朱海，王玥，汪峰峰，尹晓梅，周莉，汪奇志，张世清，汪天平*   

  1. 230061合肥市，安徽省血吸虫病防治研究所，*通讯作者
  • 出版日期:2017-03-10 发布日期:2017-04-12
  • 基金资助:

    国家科技重大专项（2012ZX10004-220，2008ZX10004-011）

Longevity of Schistosoma japonicum cercariae with infectivity and egg release in excreta in the infected mice

Sun Chengsong, Zhu Hai, Wang Yue, Wang Fengfeng, Yin Xiaomei, Zhou Li, Wang Qizhi, Zhang Shiqing, Wang Tianping*   

  1. Anhui Provincial Institute of Schistosomiasis Control, Hefei 230061, China.  *Corresponding Author.
  • Online:2017-03-10 Published:2017-04-12

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摘要： 目的 探索感染小鼠的血吸虫尾蚴时龄增加至何水平时，小鼠不随粪便排出虫卵。 方法 取新鲜逸出的尾蚴20条置于盛有去氯水的短试管中，20~25℃恒温放置2~72 h后，采用尾部浸泡法感染小鼠，感染小鼠饲养42 d，连续3天收集粪便，镜检判定粪便中血吸虫虫卵排出情况，浓集法计数每克小鼠粪便虫卵数量（EPG）。最后解剖实验小鼠，灌注法收集成虫，计算小鼠感染率及体内虫负荷水平。 结果 当感染尾蚴的时龄增加至24 h，12只小鼠中仍有1只在感染后42~44 d随粪便排出虫卵，而当感染尾蚴的时龄增加至48 h，12只小鼠中未出现成功感染的小鼠。随着感染尾蚴时龄的增加，粪便中排出虫卵的小鼠数量减少。 结论 血吸虫尾蚴在水体中虽不摄取营养，但尾蚴体内储存的能量足以维持其自钉螺逸出后24h内成功感染小鼠并使其随粪便排出虫卵，从而使血吸虫生活史得以延续。

关键词: 日本血吸虫, 尾蚴时龄, 粪便虫卵, 小鼠

Abstract:

Objective To observe when the cercariae of Schistosoma japonicum attacks will not induce eggs in the feces of the infected mice. Methods Twenty newly escaped cercariae were obtained and stored in a short test tube with dechlorinated water, and incubated for 2 to 72 h at constant 20~25℃. The mice were infected by tail immersion method, and fed for 42 days. Then feces of mice were collected in consecutive 3 days, and microscopically detected for the egg release in the excreta. Concentration method was used to calculate the eggs per gram (EPG) in mice stools. Finally, laboratory mice were dissected for harvesting the adult worms by perfusion technique, and the infection rate as well as worm burden was calculated. Results Eggs of Schistosoma japonicum occurred in the excreta following infection with the cercariae 42 to 44 days in 1 of the 12 mice by the longevity of the cercariae in 24 h, yet no infection was found in the 12 mice as the cercariae survival was increased to 48 h, and egg release with the feces tended to decrease as increase of the longevity of the cercariae. Conclusion Although the cercariae of Schistosoma japonicum have no capacity to absorb nutrition in the water, yet the energy in cercariae is sufficiently to sustain the infection with the mice 24 h after escaping from the snails, leading to egg release with the feces in the infected mice and extension of the life cycle of Schistosoma japonicum.

Key words: Schistosoma japonicum, Cercariae longevity, Fecal eggs, Mice