热带病与寄生虫学

热带病与寄生虫学 ›› 2016, Vol. 14 ›› Issue (3): 125-127,132.doi: 10.3969/j.issn.1672-2302.2016.03.001

• 论著 •    下一篇

日本血吸虫抗体检测试剂盒（IHA法）早期诊断效果的初步评估

孙成松，章乐生，朱海，王玥，汪峰峰，周莉，尹晓梅，汪奇志，张世清，汪天平   

  1. 230061 合肥市，安徽省血吸虫病防治研究所
  • 出版日期:2016-09-10 发布日期:2016-09-30
  • 基金资助:

    国家科技重大专项（2012ZX10004-220，2008ZX10004-011）

Preliminary evaluation of an indirect hemagglutination assay kit on early diagnosis of schistosomiasis japonica

Sun Chengsong, Zhang Lesheng, Zhu Hai, Wang Yue, Wang Fengfeng, Zhou Li, Yin Xiaomei, Wang Qizhi, Zhang Shiqing, Wang Tianping   

  1. Anhui Provincial Institute of Schistosomiasis Control, Hefei 230061, China
  • Online:2016-09-10 Published:2016-09-30

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摘要： 目的  初步评估日本血吸虫抗体检测试剂盒（IHA法）的早期诊断效果。 方法  建立日本血吸虫感染家兔模型，日本血吸虫抗体检测试剂盒（IHA法）及基于该试剂盒所使用的血吸虫天然蛋白抗原X的ELISA法 (抗原X-ELISA法) 检测感染前及感染后第1、2、3、4、5、6周兔血清，并比较两种方法的早期诊断效果。 结果  日本血吸虫抗体检测试剂盒（IHA法）检测感染前及感染后第1、2周兔血清的阳性率均为0，从感染后第3w开始阳性率均达到100%；抗原X-ELISA法检测日本血吸虫感染前正常兔血清的假阳性率为30.77%，检测感染后第1、2周兔血清的阳性率分别为0和16.67%，从感染第3周开始阳性率也达到100%。两种方法检测同一时间点兔血清的阳性率差异均无统计学意义（P＞0.05）。 结论  日本血吸虫抗体检测试剂盒（IHA法）在早期诊断日本血吸虫病方面具有一定的潜在价值。

关键词: 日本血吸虫病, 间接血凝试验（IHA）, 酶联免疫吸附试验, 早期诊断

Abstract:

Objective To preliminarily evaluate an indirect hemagglutination(IHA) assay kit for early diagnosis of schistosomiasis japonica. Methods Rabbit models were infected with Schistosoma japonicum. The sera were collected from infected and non-infected rabbits on week 1, 2, 3, 4, 5 and 6, respectively, after infection, and subjected to detection using IHA assay kit and enzyme-linked immunosorbent assay(ELISA) based on the antigen X (X-ELISA). Then the two methods were compared regarding the outcomes in early diagnosis of schistosomiasis japonica. Results IHA assay kit revealed 0% positive serum from non-infected rabbits and from infected rabbits in the 1st and 2nd week after infection and 100% positive results from the 3rd week after infection. Contrarily, X-ELISA indicated false positive rate of 30.77% in the sera of non-infected rabbits, and 0% and 16.67% positive results in the 1st and 2nd week after infection, and 100% positive results by the 3rd week. The difference was not significant regarding the positive rate measures at the same time point by two techniques(P>0.05). Conclusion IHA assay kit has potential value in early diagnosis of schistosomiasis japonica.

Key words: Schistosomiasis japonica, Indirect hemagglutination assay, Enzyme-linked immunosorbent assay, Early diagnosis