Journal of Tropical Diseases and Parasitology

Journal of Tropical Diseases and Parasitology ›› 2014, Vol. 12 ›› Issue (4): 187-189,193.doi: 10.3969/j.issn.1672-2302.2014.04.001

    Next Articles

Characteristics of rpoB and katG gene mutation in drug-resistant Mycobacterium tuberculosis in Lu'an area

Chang Hongwei1 Li Kaichun1, Ma Tai1, Yang Wei1, Zhangfeng1, Zhang Limei1, Sun Lin2, Li Juan3, Li Wei4   

  1. 1.Lu’an Municipal Center for Disease Control and Prevention, Lu'an 237000, China. 2. Center for Disease Control and Prevention of Jin'an District. 3. Center for Disease Control and Prevention of Yu'an District. 4. Center for Disease Control and Prevention of Huoshan County, Huoshan 237000,China
  • Online:2014-12-10 Published:2015-01-27

Abstract:

Objective To investigate the characteristics of mutations in the rpoB and katG genes(clinical isolates) leading to drug- resistance in Mycobacterium tuberculosis(M. tuberculosis) in Lu’an area. Methods Drug sensitive test was performed in 65 strains of M. tuberculosis clinically isolated from the sputum samples collected in Lu'an area, and the gene fragments of rpoB and katG were amplified with specific primers, sequenced and analyzed. Results Of the 60 strains of M. tuberculosis, 9 were mutations involved in resistance against isoniazid, 14 against rifampin, and 6 against both. The mutation for the 4 in 9 isoniazid-resistant strains(katG gene) occurred at codon 315(codon change from AGG to ACC or ACA, 44.4%), and 11 of 14 rifampin resistant strains(rpoB gene) occurred at codon 516(GAC→GTC), 526(CAC→CGC or TAC) and 531(TCG→TTG,78.6 %). Five in 6 strains resistant to both isoniazid and rifampin had mutations, which accounted for 83.3%.Conclusion Drug-resistance M. tuberculosis in Lu’an area is associated with katG and rpoB gene mutation,in which the katG gene primarily occurs at codon 315(AGC→ACC or ACA), and the rpoB at 516(GAC→GTC), 526(CAC→CGC or TAC) and 531(TCG→TTG).

 

Key words: Mycobacterium tuberculosis, Drug resistance, katG, rpoB, Mutation