Journal of Tropical Diseases and Parasitology ›› 2013, Vol. 11 ›› Issue (2): 89-90.doi: 10.3969/j.issn.1672-2302.2013.02.008

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Study on methylene blue reductive enzymatic application in rapid detection on microorganism for sudden water pollution

Qiu Ying, Wang Mindi, Qiu Hemin   

  1. Langfang Center for Disease Control and Prevention,Langfang 065000, China.
  • Online:2013-02-25 Published:2013-06-08

Abstract:

Objective To study the feasibility that methylene blue reductase method to the rapid detection on microorganism index in sudden water pollution. Methods The total number of colonies was tested by the comparison between the fading time of methylene blue and the GB/T5750.12-2006 plate count method. Results There was an inverse relationship between the fading time of methylene blue and total number of colonies. The more the number of colonies, the shorter the fading time of methylene blue. Conclusion Methylene blue reduction method is simple, rapid, accurate, reliable and can be applied to rapid detection of the total bacterial colonies in sudden water pollution.

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