Abstract:

Objective To investigate the association of p53 mRNA expression level with clinic pathological characteristics on patients with colorectal cancer with/without schistosomias. Methods Tissue specimens were obtained from 38 patients undergoing surgery. Real-time quantitative reverse transcriptional PCR (qRT-PCR) was performed for amplification of p53 mRNA from the 20 tumor tissues（A group: colorectal cancer with schistosomias）and 18 tumor tissues (B group: colorectal cancer without schistosomias）. Results P53 mRNA expression was detected in all tissues, and the level of p53 mRNA was significantly higher in group A than in group B. The level of p53 mRNA was highly correlated with tumor size and lymph node metastasis, but not with the patient's sex and age. Conclusion The level of p53 mRNA was correlated to metastasis of colorectal cancer. The schistosome infection may have some influence on expression of gene p53 in colorectal cancer.

Key words: Colorectal cancer, Schistosome infection, Gene p53, qRT-PCR