热带病与寄生虫学

热带病与寄生虫学 ›› 2015, Vol. 13 ›› Issue (2): 86-87,111.doi: 10.3969/j.issn.1672-2302.2015.02.008

• 论著 • 上一篇    下一篇

Kato-Katz与醛醚法定性定量诊断血吸虫病的对比分析

阳桂芬，卜开明，吴昭武，彭喜松，符应林，周泗罗，刘启立   

  1. 414000 湖南岳阳市，湖南省血吸虫病防治所，湖南省血吸虫病免疫与传播控制重点实验室，WHO湖区血吸虫病防治研究合作中心（阳桂芬、卜开明、吴昭武、彭喜松、刘启立）；湖南省沅江市血吸虫病防治院（符应林、周泗罗）
  • 出版日期:2015-06-10 发布日期:2015-07-10

Comparison of Kato-Katz and formol-ether concentration method in diagnosis of schistosomiasis japonica

Yang Guifen1, Bu Kaiming1, Wu Zhaowu1, Peng Xisong1, Fu Yinglin2, Zhou Siluo2, Liu Qili1   

  1. 1. Hunan Institute of Schistosomiasis Control, Hunan Key Laboratory of Immunology and Transmission Control on Schistosomiasis, WHO Collaboration Center for Schistosomiasis in Lake Region, Yueyang 414000,China; 2. Yuanjiang Hospital of Anti-Schistosomiasis, Yuanjiang 413100, China.
  • Online:2015-06-10 Published:2015-07-10

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摘要： 【摘要】 目的  评价Kato-Katz与醛醚法检测日本血吸虫病定性与定量的效果。 方法  以Kato-Katz与醛醚法联合检出的38例日本血吸虫卵阳性患者的结果为金标准，将Kato-Katz与醛醚法的检测结果分别与金标准进行比较。 结果  Kato-Katz与醛醚法的虫卵阳性检出率分别为81.58%和89.47%，经四格表的确切概率法检验，差异无统计学意义(P＝0.795)；阳性患者的平均EPG，Kato-Katz法为醛醚法的3.6倍；但Kato-Katz法的材料费与标本制作费比醛醚法低2.33倍。 结论  日本血吸虫卵的检出率Kato-Katz与醛醚法相近，但血吸虫病感染度，Kato-Katz法的EPG值远高于醛醚法，在进行血吸虫病的定量研究时，应采用醛醚法为宜。

关键词: 日本血吸虫病, Kato-Katz法, 醛醚法, 检出率, 感染度

Abstract:

【Abstract】 Objective  To evaluate the qualitative and quantitative effect of Kato-Katz and formol-ether concentration methods in diagnosis of Schistosomiasis japonica. Methods  Kato-Katz and formol-ether concentration methods were respectively used to determine the findings by comparing with the positive results of Schistosoma japonicum eggs detected by the combined use of the two approaches in 38 cases as gold standard. Results Positive egg rate by Kato-Katz and formol-ether concentration technique was respectively 89.47% and 81.58%, and the difference was not significant after verification of the exact probability by fourfold and contingency table (P = 0.795). By mean eggs per gram(EPG) of stool from the positively infected patients, the count by Kato-Katz method was 3.6 times of that by formol-ether concentration technique, and the cost of materials and specimen preparation was 2.33 times lower in Kato-Katz method than formol-ether concentration. Conclusion  Kata-Katz method has similar detection rate to formol-ether concentration for Schistosoma japonicum eggs, yet the former can result in higher EPG value in determination of the infection density than the latter. Given quantitative study of schistosomiasis, formol-ether concentration is appropriately recommended.

Key words: Schistosomiasis japonica, Kato-Katz method, Formol-ether concentration technique, Detective rate,  , Infectiosity