热带病与寄生虫学

热带病与寄生虫学 ›› 2016, Vol. 14 ›› Issue (3): 141-144.doi: 10.3969/j.issn.1672-2302.2016.03.005

• 论著 • 上一篇    下一篇

2015年重庆市3例输入性卵形疟原虫wallikeri亚种感染病例的实验室诊断

谭妍，李志峰，凌华，周杨，许静茹   

  1. 400042  重庆市，重庆市疾病预防控制中心
  • 出版日期:2016-09-10 发布日期:2016-09-30

Laboratory diagnosis on three cases of imported Plasmodium ovale wallikeri infection in Chongqing in 2015

Tan Yan, Li Zhifeng, Lin Hua, Zhou Yang, Xu Jingru   

  1. Chongqing Center  for Disease Control and Prevention, Chongqing 400042, China
  • Online:2016-09-10 Published:2016-09-30

PDF (PC)

1255

被引次数

3

摘要： 目的   分析重庆市2015年3例输入性卵形疟原虫wallikeri亚种感染病例病原、抗原及核酸检测过程，减少卵形疟原虫的漏诊和误诊。 方法  收集流行性病学资料及血样，按照WS259-2015疟疾的诊断标准对3例血样进行血涂片镜检、快速疟疾诊断试纸条检测（RDT）及巢式PCR检测。 结果  3例患者均为非洲务工归国人员，并都有疟疾发病史。血涂片镜检结果，3例血样均为疟原虫阳性。RDT检测结果3例均为除恶性疟以外其他疟原虫阳性。国家参比实验室推荐的巢式PCR检测DNA，3例均为阴性。用卵形疟原虫wallikeri亚种的种特异性引物的PCR检测，3例均为阳性。 结论  根据镜检、RDT和卵形疟原虫wallikeri亚种种特异性PCR检测确定，重庆市2015年首次发现了3例卵形疟原虫wallikeri亚种感染输入病例，为重庆地区首次发现。

关键词: 卵形疟原虫wallikeri亚种, 巢式PCR, 实验室诊断, 重庆市

Abstract:

Objective  To analyze the laboratory processes in detecting the antigen and DNA for diagnosis of three imported cases of Plasmodium ovale wallikeri infection in order to reduce the misdiagnosis and missed diagnosis of tertian malaria in humans. Methods  Epidemiological information and blood samples were collected from the three patients, and the blood samples were detected by microscopy, Rapid Diagnostic Test（RDT）and nested PCR according to the Diagnostic Criteria for Malaria(WS259-2006). Results  The three patients were returned workers from Africa, with a history of malaria. Microscopic examination indicated positive blood film for the three cases, and RDT eliminated Plasmodium falciparum infection. Nested PCR for detecting the DNA in Plasmodium ovale wallikeri by using specific primers also indicated positive results. Conclusion  Three cases of Plasmodium ovale wallikeri infection were identified by combined confirmation with microscopy, RDT and gene sequencing. These are the first imported tertian malaria in Chongqing in 2015.

Key words: Plasmodium ovale wallikeri, Nested PCR, Laboratory diagnosis, Chongqing City