Metagenomic analysis of symbiotic microbiota in Aedes albopictus from Songjiang District, Shanghai Municipality

doi:10.20199/j.issn.1672-2302.2024.06.003

Abstract

Abstract:

ObjectiveTo preliminarily investigate the species diversity and functional variations of the symbiotic microflora in Aedes albopictus within the field environment of Songjiang District, Shanghai Municipality for understanding the changes of disease spectrum transmitted by Ae. albopictus and providing new ideas for biological control of Ae. albopictus. Methods Between July and September 2023, we harvested the Ae. albopictus samples by human trap method from the three field sampling sites in Guangfulin Country Park, Sheshan National Forest Park and Chenshan area, Songjiang District, Shanghai. After morphological identification of the specimens, DNA was extracted, and metagenomic sequencing technology was used to annotate the sample species. Functional annotation was performed through the Comprehensive Antibiotic Resistance Database (CARD) and Carbohydrate-Active enZymes Database (CAZy). Results In this investigation, we successfully captured 417 heads of Ae. albopictus in total, and annotated 236 bacterial genera and 23 viral genera, of which 52.97% (125/236) of the bacterial genera and 21.73% (5/23) of the viral genera were shared in the Ae. albopictus from 3 sampling sites. The dominant phyla of the symbiotic microbiota were Pseudomonadota and Bacillota, with Wolbachia, Streptococcus and Enterobacter showing the higher abundance. Exclusion of the unclassifiable microbial virus phylum, the prevailing virus phyla in the sampls consisted of Negarnaviricota and Peploviricota. Genera with relatively higher abundance included Alphanudivirus and Orthoflavivirus. Annotating the antibiotic resistance genes via CARD database showed that the abundance of fabG, abcA, rpoB and other pathways were higher. Annotation of the function of carbohydrate active enzymes based on CAZy database revealed higher abundance of glycosyl transferases (GT), glycoside hydrolases (GH) and polysaccharide lyases (PL). Conclusion The composition of symbiotic microbiota in Ae. albopictus in Songjiang District of Shanghai is highly similar and the core symbiotic microbiota remains stable. The symbiotic microbiota of Ae. albopictus is potentially associated with the drug resistance.

Key words: Aedes albopictus, Symbiotic microbiota, Metagenomics, Database, Function annotation

CLC Number:

R373.3

YE Duqiu, ZHANG Chi, PANG Bowen, WU Jialing, JIANG Lufang, LÜ Xihong. Metagenomic analysis of symbiotic microbiota in Aedes albopictus from Songjiang District, Shanghai Municipality[J]. Journal of Tropical Diseases and Parasitology, 2024, 22(6): 334-338.

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采样点	采样点经纬度	采样时间
广富林	（31.069 203N，121.183 424E）	2023年7—9月
佘山	（31.094 166N，121.196 041E）	2023年7—8月
辰山	（31.075 000N，121.183 040E）	2023年8—9月

采样点	样本数（只）	原始读段数	清洗后读段数	读段长度（nt）	Q20（%）	GC含量（%）
广富林	136	92 876 170	91 253 886	150.00	97.70	40.76
佘山	143	100 300 226	91 312 476	150.00	97.96	40.80
辰山	138	85 520 800	85 296 178	150.00	97.53	40.81
合计	417	278 697 196	267 862 540	150.00	97.73	40.79