Abstract:

Objective To observe the effect of Lysophosphatidyl choline (LPC) on Renin-angiotensin system (RAS) of human umbilical vein endothelial cells (HUVECs). Methods HUVECs were cultured in vitro. The study was designated to 4 groups: (1) normal control, (2) low-concentration LPC group (10μg/L),(3) middle-concentration LPC group (20μg/L), (4) high-concentration LPC group（40μg/L）.Expression of angiotensinⅡ (AngⅡ ) and AngⅡ type1 receptor(AT1R), AngⅡ type2 receptor(AT2R) mRNA were observed by radio-immunity and RT-PCR, respectively. Results Compared with normal control group, LPC could significantly up- regulate the expression of AngⅡ and AT1R mRNA. Conclusion LPC could partly up-regulate the expression of RSA of human umbilical vein endothelial cells.

Key words: Lysophosphatidyl choline, Human umbilical vein endothelial cells, Renin- angiotensin system（RAS）