Molecular epidemiological investigation of Bartonella infection in small mammals in Xishuangbanna Prefecture, Yunnan Province

doi:10.20199/j.issn.1672-2302.2024.06.004

Abstract

Abstract:

Objective To understand the status of Bartonella infection in small mammals living in Xishuangbanna Dai Autonomous Prefecture (referred to as Xishuangbanna Prefecture), Yunnan Province, for scientific evidences in local prevention and control of Bartonellosis. Methods Between 2021 and 2023, we captured small mammals in different environments in Menghai County, Mengla County, and Jinghong City of Xishuangbanna Prefecture, Yunnan Province. After morphological identification, the hearts, lives, spleens, and lungs were obtained, in which DNA was extracted to undergo amplification of the gltA gene fragment of Bartonella using PCR. Then the sequences of the positive PCR products were subsequently determined, and the homology and phylogenetic analysis were performed in the sequences obtained. Results A total of 219 small mammals representing 17 species were captured, including 193 rodents in 6 genera and 11 species, and 26 insectivores in 4 genera and 6 species. Among the captured small mammals, Rattus flavipectus was the most common (65.75%, 144/219), followed by Eothenomys eleusis (6.39%, 14/219), Mus pahari (4.57%, 10/219) and Crocidura dracula (4.57%, 10/219). A total of 29 small mammals were found to carry the target gene fragment, with a Bartonella infection rate of 13.24% (29/219). The infection rate was 14.58% (21/144), 7.14% (1/14), 40.00% (4/10), 22.22% (2/9) and 25.00% (1/4), respectively for Rattus flavipectus, Eothenomys eleusis, Mus pahari, Mus caroli and Niviventer fulvescens. By sequence alignment and analysis, 8 species of Bartonella were identified, in which 14 strains were associated with B. tribocorum, 7 strains with B. Queenslandensis, 2 strains with B. phoceensis, 2 strains with B. elizabethae, 1 strain with B. Kosoyi, 1 strain with B. Taylorii, 1 strain with B. Rochalimae, and 1 strain with B. sylvatica. Conclusion Bartonella infections are found in the small mammals living in the areas of Xishuangbanna Prefecture, Yunnan Province, and are characterized by diverse genotypes and hosts.

Key words: Bartonella, gltA gene, Small mammals, Xishuangbanna Prefecture

CLC Number:

R516.8，R184.3

LUO Zhi, YANG Hongmei, LI Dongmei, LI Miao, DU Chaobo, XIANG Rong, CAO Zhanxing, DU Chunhong. Molecular epidemiological investigation of Bartonella infection in small mammals in Xishuangbanna Prefecture, Yunnan Province[J]. Journal of Tropical Diseases and Parasitology, 2024, 22(6): 339-345.

Figures/Tables 4

Table 1

Table 2

Table 3

Figure 1

References 25

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目	科	属	种	捕获数（只）	感染数（只）	感染率（%）	感染的巴尔通体种
啮齿目	鼠科	家鼠属	黄胸鼠	144	21	14.58	特利波契巴尔通体、昆州巴尔通体、菲西巴尔通体（B. phoceensis）、伊丽莎白巴尔通体、B. kosoyi
			斯氏家鼠	5	0	0
			褐家鼠	3	0	0
		小鼠属	锡金小鼠	10	4	40.00	昆州巴尔通体、罗莎利马巴尔通体（B. rochalimae）
			卡氏小鼠	9	2	22.22	昆州巴尔通体
			小家鼠	1	0	0
		白腹鼠属	针毛鼠	4	1	25.00	森林巴尔通体（B. silvatica）
			北社鼠	1	0	0
		板齿鼠属	板齿鼠	1	0	0
		硕鼠属	青毛鼠	1	0	0
	仓鼠科	绒鼠属	滇绒鼠	14	1	7.14	泰勒巴尔通体
食虫目	鼩鼱科	麝鼩属	长尾大麝鼩	10	0	0
			灰麝鼩	5	0	0
			中麝鼩	2	0	0
		微尾鼩属	四川短尾鼩	4	0	0
		臭鼩属	臭鼩鼱	1	0	0
	猬科	毛猬属	毛猬	4	0	0
合计				219	29	13.24

调查点	小型兽类种类	捕获数（只）	感染不同巴尔通体的小型兽类数（只）									感染率（%）
调查点	小型兽类种类	捕获数（只）	特利波契巴尔通体	昆州巴尔通体	菲西巴尔通体	伊丽莎白巴尔通体	罗莎利马巴尔通体	森林巴尔通体	泰勒巴尔通体	B. kosoyi	合计	感染率（%）
勐海	黄胸鼠	37	2	1	0	0	0	0	0	0	3	8.10
	滇绒鼠	14	0	0	0	0	0	0	1	0	1	7.14
	锡金小鼠	9	0	3	0	0	1	0	0	0	4	44.44
	长尾大麝鼩	9	0	0	0	0	0	0	0	0	0	0
	卡氏小鼠	6	0	2	0	0	0	0	0	0	2	33.33
	四川短尾鼩	4	0	0	0	0	0	0	0	0	0	0
	灰麝鼩	4	0	0	0	0	0	0	0	0	0	0
	毛猬	2	0	0	0	0	0	0	0	0	0	0
	臭鼩鼱	1	0	0	0	0	0	0	0	0	0	0
	青毛鼠	1	0	0	0	0	0	0	0	0	0	0
	北社鼠	1	0	0	0	0	0	0	0	0	0	0
	斯氏家鼠	1	0	0	0	0	0	0	0	0	0	0
	小家鼠	1	0	0	0	0	0	0	0	0	0	0
	针毛鼠	1	0	0	0	0	0	0	0	0	0	0
	小计	91	2	6	0	0	1	0	1	0	10	10.99
勐腊	黄胸鼠	25	2	0	0	0	0	0	0	0	2	8.00
	斯氏家鼠	4	0	0	0	0	0	0	0	0	0	0
	卡氏小鼠	3	0	0	0	0	0	0	0	0	0	0
	针毛鼠	3	0	0	0	0	0	1	0	0	1	33.33
	毛猬	2	0	0	0	0	0	0	0	0	0	0
	中麝鼩	2	0	0	0	0	0	0	0	0	0	0
	板齿鼠	1	0	0	0	0	0	0	0	0	0	0
	灰麝鼩	1	0	0	0	0	0	0	0	0	0	0
	锡金小鼠	1	0	0	0	0	0	0	0	0	0	0
	长尾大麝鼩	1	0	0	0	0	0	0	0	0	0	0
	小计	43	2	0	0	0	0	1	0	0	3	6.98
景洪	黄胸鼠	82	10	1	2	2	0	0	0	1	16	19.51
	褐家鼠	3	0	0	0	0	0	0	0	0	0	0
	小计	85	10	1	2	2	0	0	0	1	16	18.82
合计		219	14	7	2	2	1	1	1	1	29	13.24

标本编号	巴尔通体种类	参考序列号	同源性（%）
JH-6	伊丽莎白巴尔通体	JX158352.1	100.00
JH-11	菲西巴尔通体	GU056197.1	100.00
JH-19	特利波契巴尔通体	KT324541.1	100.00
JH-39	特利波契巴尔通体	KT324541.1	100.00
JH-53	昆州巴尔通体	JX158357.1	97.70
JH-56	特利波契巴尔通体	KT324541.1	100.00
JH-84	特利波契巴尔通体	KT324541.1	100.00
JH-86	伊丽莎白巴尔通体	GU056192.1	100.00
JH-90	B. kosoyi	OP382453.1	100.00
JH-91	特利波契巴尔通体	KT324541.1	100.00
JH-94	菲西巴尔通体	OP375127.1	99.70
JH-119	特利波契巴尔通体	KT324541.1	100.00
JH-135	特利波契巴尔通体	KT324541.1	100.00
JH-157	特利波契巴尔通体	KT324541.1	100.00
JH-164	特利波契巴尔通体	KT324541.1	100.00
JH-166	特利波契巴尔通体	KT324541.1	100.00
MH-1	特利波契巴尔通体	KT324541.1	100.00
MH-3	特利波契巴尔通体	KT324541.1	100.00
MH-37	罗莎利马巴尔通体	PP466317.1	96.50
MH-60	昆州巴尔通体	JX158357.1	93.90
MH-65	昆州巴尔通体	JX158357.1	93.90
MH-78	昆州巴尔通体	JX158357.1	96.80
MH-85	昆州巴尔通体	JX158357.1	95.90
MH-124	昆州巴尔通体	JX158357.1	93.90
MH-174	泰勒巴尔通体	KT445921.1	96.10
MH-177	昆州巴尔通体	JX158357.1	93.90
Mla-48	特利波契巴尔通体	KT324541.1	100.00
Mla-60	特利波契巴尔通体	KT324541.1	100.00
Mla-78	森林巴尔通体	AB242287.1	98.20