Journal of Tropical Diseases and Parasitology ›› 2021, Vol. 19 ›› Issue (6): 348-350,357.

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Advances ingenomic DNA extraction from microarthropods without morphological damage

FAN Hai-ye,CHEN Hui-lin,ZHU Yong-hang,WANG Yan,WANG Ruo-hang,ZHAO Wei,TAO Xiang-lin,SUN En-tao #br#   

  1. Medical Laboratory Science,Wannan Medical College,Wuhu 241002,Anhui Province,China
  • Online:2021-12-20 Published:2021-12-31

Abstract: DNA extraction represents the key step for DNA barcoding and the basis of subsequent field study. Common genomic DNA extraction technique may result in damage to the important external morphological nature of microarthropods due
to their smaller size,which makes it impossible to further identify the morphology. However,a method of DNA extraction
without morphological damage can effectively make up for the deficiency of the former. This paper reviewed the genomic
DNA extraction technique without morphological damage to the microarthropods,and compared the advantages and disadvantages of different genomic DNA extraction procedures for microarthropods,with an attempt to lay a foundation for further optimization,improvement and exploration of a simpler and more efficient DNA extraction technique without morphological
damage.

Key words: DNA barcoding, Microarthropods, Sampling without morphological damage, Genomic DNA extraction

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