Abstract: Accurate detection of allergens is vital for the prevention,diagnosis and treatment of allergic conditions. Currently, in vitrotechnique represents the leading research interest in allergen detection. This paper summarizes the available detection techniques targeting at serum sIgE measurement,including radioallergosorbent test,enzyme-linked immunosorbent assay,fluoroenzyme immunoassays,magnetic nano-particle chemiluminescence immunoassay,immunoblotting assay,liquidphase chip method as well as bioresonance method for direct detection of the allergens. In addition,the advantages and disadvantages are also compared pertinent to various in vitro detection techniques,with an attempt to provide reference for clinical option in selection of appropriate in vitrotechnique in detecting allergen in specific subjects.

Key words: Allergen, specific IgE, In vitro detection