Abstract:

Objective To understand the pollution status of O1/O139 group Vibrio cholerae in aquatic products and water bodies in Wuzhou City for scientific evidences in following prevention and control tasks. Methods From 2012 to 2023, we conducted random sampling of the aquatic products and aquaculture pond water from farmers' markets and supermarkets in Wuzhou City annually from May to October, and collected the pond water and river water that was susceptible to contamination at designated points. The samples were all subjected to isolation and culturing for Vibrio cholerae as well as biochemical identification, serotype and virulence gene detection. Results In total, 4 844 samples of aquatic products and water bodies were monitored in Wuzhou City from 2012 to 2023. Vibrio cholerae was detected in 53 samples of O1/O139 group, with a total detection rate of 1.09%. Of the samples, 3 020 were from aquatic products and 1 824 from water bodies. The detection rate was 1.42% (43/3 020) for the former, and 0.55% (10/1 824) for the latter, with significant difference (χ²=8.056, P<0.05). The detection rate of Vibrio cholerae in aquatic products differed significantly in different months (χ²=16.053, P<0.05), and was higher in September (3.54%, 12/339) and lower in July (0.68%, 4/588).The difference of Vibrio cholera was significant in different types of aquatic products and water bodies (χ²=82.308, 15.752, respectively, both P<0.05). Higher detection rate was seen in frogs in aquatic products (10.27%, 23/224) and in aquaculture pond water (2.43%, 7/288). In the 53 strains detected, 22 were O1 serotype Ogawa, 24 were O1 serotype Inaba, and 7 were O139 type. CT virulence genes were all negative. Conclusion Pollution of Vibrio cholerae was seen in the aquatic products and water bodies in Wuzhou City. The findings suggest that it is necessary to further strengthen the monitoring efforts, timely understand the contamination status of Vibrio cholerae, and strictly prevent the occurrence of cholera epidemic.

Key words: Vibrio cholerae, Aquatic products, Water bodies, Monitoring, Wuzhou City