Abstract:

Objective  To observe the results of hatching Fasciola eggs with triangle flask at room temperature and to experimentally test the infection status of Physa acuta with Fasciola miracidia. Methods  Active Fasciola eggs were initially isolated from the cxcreta of cattle fed in Dali area of Yunan province, and incubated by two groups(group A and B). Incubation water was daily refreshed in group A, and was not in group B. The hatching results were observed in two groups. Results Fasciola miracidia were hatched at average water temperature of 24℃ by the thirteenth day in two groups.The miracidia were more in production and easy observation in group A, whereas miracidium production was fewer and observation was harder due to liquid opacity. Full hatching of the miracidia consumed 20 to 30 days in two groups. Physa acuta were not infected with Fasciola miracidia. Conclusion  Fasciola eggs can be hatched into miracidia with triangle flask at above 22℃ in room condition during summer seasons. Daily change of the water deprived of chlorine may lead to favorable incubation and visual observation of the miracidia. Free use of incubator may reduce the cost, which is worthy of recommendation at basic level or field study. In addition. Our findings indicate that Physa acuta is not the suitable intermediate host for Fasciola, suggesting that Physa acuta infection needs further investigation.

Key words: Fasciola, Hatch, Miracidia, Physa acuta