Journal of Tropical Diseases and Parasitology

Journal of Tropical Diseases and Parasitology ›› 2015, Vol. 13 ›› Issue (4): 189-192.doi: 10.3969/j.issn.1672-2302.2015.04.002

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Preliminary study on early diagnosis of schistosomiasis japonicum by IHA based on soluble schistosomula antigen (SSA)

Sun Chengsong, Zhang Lesheng, Wang Fengfeng, Wang Yue, Yin Xiaomei, Zhou Li, Wang Qizhi, Zhang Shiqing, Wang Tianping*   

  1. Anhui Provincial Institute of Schistosomiasis Control, Hefei 230061, China
  • Online:2015-12-10 Published:2016-01-05

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Abstract:

Objective  To develop an IHA method by using soluble schistosomula antigen (SSA) for early diagnosis of schistosomiasis japonica. Methods  Schistosomula were collected 13 days post-infection and SSA were prepared. The optimal volume ratio between SSA and erythrocyte and the best blocking concentration (V/V) by negative rabbit sera of inactivating complement were explored. Then the IHA method based on SSA (SSA-IHA) was constructed and the sera from non-infected rabbits and 1, 2, 3, 4, 5, 6 weeks post-infection were detected. Results  The optimal volume ratio between SSA and erythrocyte was 1∶1 and the best blocking concentration (V/V) by negative rabbit sera of inactivating complement was 3%. The positive rate of sera from non-infected rabbits and 1, 2, 3, 4, 5, 6 weeks post-infection were 0%, 0%, 0%, 90.91%, 100%, 100%, and 100%, respectively when tested by SSA-IHA. Conclusion  The IHA method based on SSA has value on early diagnosis of schistosomiasis japonica.

Key words: Soluble schistosomula antigen (SSA), Indirect hemagglutination assay (IHA), Early diagnosis, Schistosomiasis japonicum