Abstract:

【Abstract】 Objective  To understand the influence of shellfish polysaccharide on the molecular expression of JAK-STAT transduction pathways in HepG2.2.15 cells. Methods  After treatment with shellfish polysaccharides, the expression levels of STAT1 and STAT2 mRNA in HepG2.2.15 cells in different phase were detected by reverse transcription polymerase chain reaction (RT-PCR). Results  RT-PCR results showed that the expression levels of the STAT1and STAT2 mRNAs in HepG2.2.15 cells treated with shellfish polysaccharides were significantly up-regulated as compared with the control group (P<0.05), which suggested the HepG2.2.15 could respond to the shellfish polysaccharides. Conclusion  The shellfish polysaccharide has certain inhibitory effect on hepatitis B virus(HBV), and its mechanism looks similar to the INF-α.

Key words: Shellfish polysaccharide, HepG2.2.15 cells, JAK-STAT transduction pathways