热带病与寄生虫学

热带病与寄生虫学 ›› 2022, Vol. 20 ›› Issue (4): 225-227,234.

• 防治管理 • 上一篇    下一篇

2021年湖南省血吸虫病监测点实验室检测能力评估结果分析

赵和钦豪，夏蒙，周杰，喻鑫玲，李广平，邓奕，罗振华，李胜明，王慧岚   

  1. 湖南省血吸虫病防治所，WHO湖区血吸虫病防治合作中心，血吸虫病免疫与传播控制湖南省重点实验室，国家临床重点专科，湖南岳阳414000
  • 收稿日期:2022-02-07 出版日期:2022-08-20 发布日期:2022-08-31
  • 作者简介:赵和钦豪，男，本科，主治医师，研究方向：临床医疗与疾病控制。E-mail：93764052@qq.com 夏蒙，男，本科，副主任技师，研究方向：微生物检验与血吸虫病防治。E-mail：emre5@qq.com 赵和钦豪、夏蒙同为第一作者
  • 基金资助:
    湖南省重点研发计划项目（2021SK2032）；湖南省科学技术厅科普专题项目（2021ZK4317）

Evaluation results of quality control and monitoring ability of schistosomiasis laboratories in Hunan Province in 2021 

ZHAO He-qin-hao, XIA Meng, ZHOU Jie, YU Xin-ling, LI Guang-ping, DENG Yi, LUO Zhen-hua, LI Sheng-ming, WANG Hui-lan#br#   

  1. Hunan Provincial Institute of Schistosomiasis Control; WHO Cooperation Center for Schistosomiasis Control and Prevention in Lake Region; Hunan Provincial Key Laboratory of Schistosomiasis Immunization and Transmission Control; National Key Clinical Specialty, Yueyang 414000, Hunan Province, China
  • Received:2022-02-07 Online:2022-08-20 Published:2022-08-31

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摘要： 目的 评估湖南省血吸虫病监测点实验室的检测能力，为完善湖南省血吸虫病监测体系能力建设提供参考。方法 在湖南省41个血吸虫病流行县的57个监测点实验室中分层抽取12个诊断网络实验室，以标准质控品考核的方式进行检测能力评价。质控品包含血清、粪样加藤厚涂片和虫卵悬液，考核内容包括间接红细胞凝集试验对血清进行定性和效价测定、改良加藤厚涂片法对粪样进行虫卵鉴定并计数、尼龙绢集卵孵化法对虫卵悬液进行定性检测，将检测结果与标准质控品进行比对，计算定性检测的符合率和定量检测的合格率，并将评估结果量化。结果 间接红细胞凝集试验、改良加藤厚涂片法和尼龙绢集卵孵化法定性检测结果的总符合率分别为96.67% （58/60）、83.33%（50/60）和91.67%（33/36）。间接红细胞凝集试验阳性质控品效价检测总合格率为54.17%（13/24），改良加藤厚涂片法血吸虫卵计数总合格率为30.00%（6/20）。12个诊断网络实验室有7个评估结果为优秀，2个为良好，3个为合格。结论 湖南省血吸虫病监测实验室检测能力基本满足监测工作需要，但在血清效价测定和虫卵计数等定量诊断方面仍需加强。

关键词: 血吸虫病；网络实验室；检测能力；评估；监测；湖南省 

Abstract: Objective To evaluate the detection capacity of schistosomiasis laboratories at the surveillance sites in Hunan Province for reference in improving the capacity of schistosomiasis surveillance system. Methods By stratified sampling, 12 diagnostic network laboratories were selected from the 57 laboratories designated in 41 count-level schistosomiasis epidemic areas in Hunan Province, and underwent evaluation for the detection ability using standard quality control samples that consisted of serum, Kato-Katz thick smear and egg suspension. The assessment included qualitative and titer determination of the standard sample serum by using indirect hemagglutination assay (IHA), qualification and counting of the schistosome eggs using modified Kato-Katz thick smear, and qualitative detection of the egg suspension using egg hatching method after nylon mesh bag concentration. The tested results at each county-level laboratory were compared with the standard quality control samples based on the coincidence rate and qualification rate of qualitative samples, and evaluated by final scores. Results Qualitative coincidence rate was 96.67% (58/60), 83.33% (50/60) and 91.67% (33/36), respectively for IHA, modified Kato-Katz thick smear and egg hatching method after nylon mesh bag concentration. All tested results met the quality control requirements. The total qualified rate of titer detection of the positive reference by IHA was 54.17% (13/24), and the qualified rate for egg count by modified Kato-Katz thick smear was 30.00% (6/20). Of the 12 diagnostic laboratories at surveillance sites, 7 were rated as "excellent", 2 as "good" and 3 as "qualified". Conclusion The assessment results demonstrate that the ability of schistosomiasis detection at county level in Hunan Province can basically meet the needs of the laboratory diagnosis, yet the quantitative diagnosis of schistosomiasis such as serum titer and egg count requires strengthening.

Key words: Schistosomiasis, Network laboratory, Detection capability, Assessment, Surveillance, Hunan Province

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