热带病与寄生虫学

热带病与寄生虫学 ›› 2015, Vol. 13 ›› Issue (4): 208-210,218.doi: 10.3969/j.issn.1672-2302.2015.04.007

• 论著 • 上一篇    下一篇

实时荧光定量RT-PCR法检测安徽宿州市手足口病病原体结果分析

胡雪影，张玲   

  1. 234000 安徽宿州市，宿州市疾病预防控制中心
  • 出版日期:2015-12-10 发布日期:2016-01-05

Detecting the pathogens of hand-foot-mouth disease with RT-PCR in population in Suzhou City

Hu Xueying, Zhang Ling   

  1. Suzhou Center for Diseases Prevention and Control, Suzhou 234000, China.
  • Online:2015-12-10 Published:2016-01-05

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摘要： 目的  了解宿州市手足口病肠道病毒谱及感染情况，为制订手足口病预防控制策略提供依据。 方法  采用实时荧光定量RT-PCR法对2012~2014年宿州市各县区哨点医院手足口病临床诊断病例进行病原学检测并分析。 结果  在962份样品中，肠道病毒核酸检测阳性384例，阳性率为39.92%；其中EV71、CoxA16分别为154例（占40.10%）、144例（占37.50%），其他型肠道病毒共86例（占22.40%）。384例阳性病例中，男247例，女137例，阳性检出率在性别间差异无统计学意义（χ2=0.83，P＞0.05）；1岁组和2岁组手足口病毒核酸检测阳性占全部阳性者的73.18%。在3~4月和6~7月的发病高峰期间，病毒主要以CoxA16型和EV71型毒株为主，10~12月以EV71型病毒株为主。在人口密集的萧县和埇桥区，其手足口核酸阳性检出率最高，分别为50.21%和42.18%。 结论  宿州市手足口病病原体主要是EV71和CoxA16，主要发生在3岁以下儿童，发病时间以每年3~4月和6~7月为主，应加强在流行季节的防控工作及3岁以下手足口病患儿的病情监测与治疗。

关键词: 手足口病, 实时荧光定量逆转录聚合酶链式反应, 肠道病毒71型, 柯萨奇病毒A组16型, 宿州市

Abstract:

Objective  To understand the entervirous type and infection status of hand-foot-mouth disease(HFMD)in population in Suzhou city for scientific evidence to plan preventive and control strategies for this entity. Methods  The specimens were collected from patients clinically diagnosed HFMD in the sentinel hospitals in Suzhou areas from 2012 to 2014, and detected for the pathogens with real-time fluorescence quantitative PCR (RT-PCR). Then the nucleic acid of enterovirus identified was analyzed. Results  In the 962 samples, positive nucleic acid sequence was identified in 384(39.92%), of which 154(40.10%) and 144(37.50%) were associated with EV71 and CoxA16, and the remaining 86(22.40%) were infected with other strains. Of the 384 patients, 247 were males and 137 females. The positive rate was not significant between genders(χ2=0.83, P＞0.05), yet higher positive rate for the nucleic acid sequence was found in patient group aged one years and two years(73.18%). The peak prevalence of HFMD was in March and April, June and July, during which the CoxA16 and EV71 were prominent, and EV71 dominated between October and December. The highest positive rate for nucleic acid sequence was seen the densely-populated areas, including Xiaoxian County and Yongqiao area, which accounted for 50.21% and 42.18%, respectively. Conclusion  EV71 and CoxA16 are the major pathogens causing HFMD in population in Suzhou city, and children aged 3 years or under are mostly affected. The prevalence is commonly seen in March and April, June and July, suggesting that prevention and control as well as monitoring and treatment of this disease should be strengthened in these two seasons and children of 3 years of age and younger.

Key words: Hand-Foot-Mouth disease, Real-Time PCR, EV71, Cox A16, Suzhou City