Abstract:

Objective To discover new candidate diagnosis molecules for the diagnosis of schistosomiasis, the Sj-Tsp6 protein gene of Schistosoma japonicum was cloned and expressed in the prokaryotic cell. Methods The specific primers were designed and synthesized. The target gene was amplified by PCR. The product from PCR was cloned into PMD-T vector, and then subcloned into the expression vector pET28a (+). After induced by IPTG, the cells were collected to analyze by SDS-PAGE and Western blot. Results A specific band of around 702 bp was amplified by PCR. A band of 28kDa fusion protein with the label of HIS was revealed by SDS-PAGE after the target gene subcloned into the expression vector pET28a (+). The fusion protein could be recognized by the sera of rabbit infected with schistosomiasis. Conclusion The cDNA encoding sj-Tsp6 protein of S. japanicum cloned and expressed in the prokaryotic cell provided basis for further study on value of SjTsp6 immunological diagnosis.

Key words: Schistosoma japonicum, Sj-Tsp6 protein, Gene cloning, Gene expression