Abstract:

Objective To evaluate the effect on the diagnosis of clonorchiasis by enzyme- linked immunosorbent assays (ELISA) as a supplementary method. Methods From 2009 to 2010, the patients with suspected clonorchiasis visited to institute of parasitology, Haerbin medical university were determined with the method of ELISA and Kato- katz. Statistic method of Bayesian was used to calculate the sensitivity and specificity. Results The total of 2 359 patients with suspected clonorchiasis were determined. The antibodypositive rate determined by ELISA was 40.44% (954/2 359), and the egg-positive rate found by examination of feces was 21.75% (513/2 359). The positive rate of ELISA was significantly higher than that of Kato-katz(P<0.05). The sensitivity, specificity, positive predictive value and negative predictive value of ELISA were 98.7%,76.53%, 54.66% and 99.52%, respectively. The agreement between the two methods was 0.564. Conclusion The combination of ELISA and Kato-katz could improve the diagnostic accuracy of clonorchiasis.

Key words: Clonorchiasis, Diagnosis, ELISA, Kato-Katz