Abstract:

Objective  To evaluate the effect of dot immunogold filtration assay (DIGFA), colloidal dye method (DDIA) and indirect hemagglutination test (IHA) for the detection of Schistosoma japonicun antibody in serum of residents in different endemic degree areas and in different endemic type areas in Tongling County. Methods  According to the method of cluster sampling, three villages were selected as study fields, namely Qunxin Village which was located in islet endemic areas and reached schistosomiasis transmission control criterion, Jinta Village which was located in hilly endemic areas and also reached schistosomiasis transmission control criterion, and Langkeng Village which reached schistosomiasis transmission interruption criterion. 150 residents were randomly selected in each village, and DIGFA, DDIA and IHA were used to detect the S. japomicum antibody in serum of the residents, and the detect results were analyzed.  Results By using DIGFA, DDIA and IHA to detect S. japonicum antibody in serum of residents, respectively, none positive were found in Qungxin Village, while the positive rate of of Jinta Village was 13.3%(20/150), 2.0%(3/150) and 12.0%(18/150), respectively, and the positive rate of Qunxin Village was 26.7%(40/150), 9.3%(14/150) and2 4.7%(37/150), respectively. By combined using DIGFA, DDIA and IHA to detect S. japomicum antibody in serum of the residents, the positive rate of Jinta Village and Qunxin Village was increased to 19.33%(29/150) and 36.0%(54/150), respectively.  By combined using DIGFA and IHA to detect S. japomicum antibody in serum of the residents, the positive rate of Jinta Village and Qunxin Village was increased to 17.33%(26/150) and 34.0%(51/150), respectively. Conclusion   Since Tongling County has reached schistosomiasis transmission control criterion, the infection rate and the infection degree of schistosomiasis in the residents are dropping year by year, combined using different methods can improve detection rate of S. japonicum antibody in serum of the residents.

Key words: Immune-assays, Schistosome japonicum, Antibody, Detection effect