Analysis on sera peptides from mice infected with Schistosoma japonicum by Chinese medicine Chaihu

Journal of Tropical Diseases and Parasitology ›› 2013, Vol. 11 ›› Issue (2): 68-70.

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Analysis on sera peptides from mice infected with Schistosoma japonicum by Chinese medicine Chaihu

Huang Yuzheng1*, Zhang Ying1, Yang Jing1, Xu Yongliang1, Gu Zhenhua2, Tao Yonghui1, Cao Peng2*. 1. Key Laboratory on Technology for Parasitic Diseases Prevention and Control, Ministry of Health, Jiangsu Institute of Parasitic Diseases, Wuxi 214064, China. 2. Laboratory of Cellular and Molecular Biology, Jiangsu Provincial Academy of Traditional Chinese Medicine, Nanjing 210028, China. *Corresponding author

Online:2013-02-25 Published:2013-06-08

Abstract

Abstract:

Objective To analyze the sera peptides from mice infected with Schistosoma japonicum by Chaihu, and evaluate its effect. Methods The infected mice model and the treated group were established, peptides were purified from the sera by MB-WCX kit (Bruker Daltonics GmBH), and then analyzed by MALDI-TOF-MS and ClinProTool algorithm. Results Among the mass range from 800 to 17 000 Da, expression of 2 924 Da and 8 210 Da were up-regulated and 11 690 Da down-regulated in the healthy and treated group compared with the infected group. Conclusion Chinese medicine Chaihu has the potential value in the treatment of schistosomiasis which provides theoratic basis for screening treating targets in protein level.

Key words: Schistosoma japonicum , Mass spectrometry , Serum peptide , Chaihu

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Analysis on sera peptides from mice infected with Schistosoma japonicum by Chinese medicine Chaihu

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