Abstract: Objective To investigate protective effects of resveratrol on the injury of bladder mucosa induced by Escherichia coli(E. coli) in rats. Methods Forty-five female SD rats, weighting (170±10) g were randomly divided into control group, model group and resveratrol treatment group. Rat models of injury of bladder muscosa were developed E. coli perfusion through the implanted catheter in the urethra, and rats in experimental group were ingragastrically administered with resveratrol in dose of 40 mg / kg. By the end of experiment, bladder mucosae were obtained and microscopically examined for the pathological changes, and colorimetry was used to measure the activities of super oxide dismutase ( SOD), glutathione peroxidase (GSH-Px) and catalase (CAT), malondialdehyde (MDA) and nitric oxide (NO). ELISA was performed to determine the level of interleukin-1β (IL-1β), IL-6, IL-8 and IL-10 as well as tumor necrosis factor-α (TNF-α) in the bladder homogenate, and Western blot was used to measure TGF-β1 expression. Results Resveratrol obviously resulted in decreased inflammatory cell count, MDA and NO level, increased activities of SOD, GSH-Px and CAT, down-regulated TGF-β1 expression, and elevated IL-6 and IL-10 level in the bladder mucosae. Conclusion Resveratrol can improve injury of bladder mucosa in rats induced by E. coli through enhanced anti-inflammatory and anti-oxidative effects, and reducing the fibrosis of the bladder mucosa.

Key words:  Resveratrol, Bladder mucosa, E. coli, Oxidative damage, Cytokines, Rat model