Polymorphism analysis of nad2 gene of Echinococcus multilocularis larvae isolates from human in Altay Prefecture of Xinjiang

doi:10.20199/j.issn.1672-2302.2025.04.010

Abstract

Abstract:

Objective To understand the genotype and genetic diversity of Echinococcus multilocularis larvae isolates from human in Altay Prefecture of Xinjiang, and to provide a theoretical basis for the prevention and control of alveolar echinococcosis (AE). Methods The liver lesion tissues were obtained from patients with AE in Altay Prefecture of Xinjiang who were treated in the First Affiliated Hospital of Xinjiang Medical University between 2021 and 2024, and from which the tissue DNA was extracted. The nad2 gene was amplified and sequenced by PCR. The successfully sequenced gene sequence was uploaded to the NCBI website for Blast comparison, the nad2 gene polymorphism was analyzed using DnaSP 5 software, the haplotype network diagram was drawn by NetWork 10, and the nad2 gene phylogenetic tree was established by using the neighbor-joining method in MEGA 11 software. Results A total of 36 liver lesion tissues were obtained. After PCR amplification, specific bands at 882 bp were observed in all 36 samples, which was in line with the expected size. The results of gene polymorphism showed that there were three haplotypes in nad2 gene belong to the Asian clade, with a haplotype diversity of 0.341 and nucleotide diversity of 0.000 76. The Hap1 was the main haplotype (29/36, 80.56%). The systematic evolution tree showed that the isolates have a close kinship with the reference sequences of Echinococcus multilocularis from Xinyuan County of Xinjiang (KT965464), Kazakhstan (AB461406), Poland (KY20700), Canada (MT429276), Hokkaido of Japan (AB461407) and Sichuan (AB461408). Conclusion Human Echinococcus multilocularis isolated from subjects in Altay Prefecture of Xinjiang belongs to Asian clade, and is genetically relatively conserved. The dominant haplotype of it is Hap1.

Key words: Echinococcus multilocularis larvae, Nad2 gene, Gene polymorphism, Altay Prefecture of Xinjiang

CLC Number:

R183.5

ZHAO Jiangshan, GUO Baoping, SHI Guangzhong, YALIKUN Maimaitiyiming, ADILI Simayi, WANG Shuo. Polymorphism analysis of nad2 gene of Echinococcus multilocularis larvae isolates from human in Altay Prefecture of Xinjiang[J]. Journal of Tropical Diseases and Parasitology, 2025, 23(4): 245-248.

Figures/Tables 3

References 22

[1]	徐凯, 王海久, 张丽, 等. 多房棘球蚴对宿主肝细胞损害机制的研究进展[J]. 中国寄生虫学与寄生虫病杂志, 2021, 39(2):256-260. doi: 10.12140/j.issn.1000-7423.2021.02.020
[2]	Knapp J, Gottstein B, Saarma U, et al. Taxonomy, phylogeny and molecular epidemiology of Echinococcus multilocularis: From fundamental knowledge to health ecology[J]. Vet Parasitol, 2015, 213(3/4):85-91.
[3]	Umhang G, Bastid V, Avcioglu H, et al. Unravelling the genetic diversity and relatedness of Echinococcus multilocularis isolates in Eurasia using the EmsB microsatellite nuclear marker[J]. Infect Genet Evol, 2021,92:104863.
[4]	马增光. 新疆昭苏盆地野生宿主多房棘球蚴病的流行病学调查[D]. 乌鲁木齐: 新疆农业大学, 2014.
[5]	Guo BP, Zhang ZZ, Guo YZ, et al. High endemicity of alveolar echinococcosis in Yili Prefecture,Xinjiang Autonomous Region,the People’s Republic of China: Infection status in different ethnic communities and in small mammals[J]. PLoS Negl Trop Dis, 2021, 15(1):e0008891.
[6]	Nakao M, Xiao N, Okamoto M, et al. Geographic pattern of genetic variation in the fox tapeworm Echinococcus multilocularis[J]. Parasitol Int, 2009, 58(4):384-389.
[7]	Vanhove MPM, Briscoe AG, Jorissen MWP, et al. The first next-generation sequencing approach to the mitochondrial phylogeny of African monogenean parasites (Platyhelminthes: Gyrodactylidae And Dactylogyridae)[J]. BMC Genomics, 2018, 19(1):520. doi: 10.1186/s12864-018-4893-5 pmid: 29973152
[8]	Clement M, Posada D, Crandall KA. TCS a computer program to estimate gene genealogies[J]. Mol Ecol, 2000, 9(10):1657-1659. doi: 10.1046/j.1365-294x.2000.01020.x pmid: 11050560
[9]	Guo BP, Zhang ZZ, Zheng XT, et al. Prevalence and molecular characterization of Echinococcus granulosus sensu stricto in northern Xinjiang, China[J]. Korean J Parasitol, 2019, 57(2):153-159.
[10]	Rozas J, Ferrer-Mata A, Sánchez-DelBarrio JC, et al. DnaSP 6: DNA sequence polymorphism analysis of large data sets[J]. Mol Biol Evol, 2017, 34(12):3299-3302. doi: 10.1093/molbev/msx248 pmid: 29029172
[11]	魏玉环, 刘华, 李武军, 等. 西藏阿里地区细粒棘球蚴人体分离株nad1基因多态性分析[J]. 中国寄生虫学与寄生虫病杂志, 2020, 38(1):17-21,29. doi: 10.12140/j.issn.1000-7423.2020.01.003
[12]	Uakhit R, Smagulova A, Syzdykova A, et al. Genetic diversity of Echinococcus spp. in wild carnivorous animals in Kazakhstan[J]. Vet World, 2022, 15(6):1489-1496.
[13]	尚婧晔, 张光葭, 喻文杰, 等. 多房棘球绦虫遗传多态性研究进展[J]. 中国寄生虫学与寄生虫病杂志, 2020, 38(5):637-641,646. doi: 10.12140/j.issn.1000-7423.2020.05.017
[14]	郭永忠, 初伊明, 阿尔新·卡德尔, 等. 新疆伊犁地区尼勒克县人群泡球蚴感染病例调查及nad2、cob基因片段分析[J]. 中国人兽共患病学报, 2017, 33(12):1066-1070. doi: 10.3969/j.issn.1002-2694.2017.12.003
[15]	尚婧晔, 张光葭, 喻文杰, 等. 中国西北部地区多房棘球绦虫cox1基因多态性研究[J]. 中国病原生物学杂志, 2021, 16(2):137-142.
[16]	Alvarez Rojas CA, Kronenberg PA, Aitbaev S, et al. Genetic diversity of Echinococcus multilocularis and Echinococcus granulosus sensu lato in Kyrgyzstan: The A2 haplotype of E. multilocularis is the predominant variant infecting humans[J]. PLoS Negl Trop Dis, 2020, 14(5):e0008242.
[17]	Bohard L, Lallemand S, Borne R, et al. Complete mitochondrial exploration of Echinococcus multilocularis from French alveolar echinococcosis patients[J]. Int J Parasitol, 2023, 53(10):555-564. doi: 10.1016/j.ijpara.2023.03.006 pmid: 37148987
[18]	Guo BP, Cairen, Wu JY, et al. The A2 haplotype of Echinococcus multilocularis is the predominant variant infecting humans and dogs in Yili Prefecture, Xinjiang[J]. Infect Genet Evol, 2024,119:105581.
[19]	赵江山, 王蒴, 张月, 等. 新疆南疆地区小型啮齿动物多房棘球蚴感染现状与基因型[J]. 中国热带医学, 2025, 25(1):46-51. doi: 10.13604/j.cnki.46-1064/r.2025.01.08
[20]	吴川川. 中国西部地区多房棘球绦虫mtDNA基因多态性分析[D]. 乌鲁木齐: 新疆农业大学, 2016.
[21]	王旭, 候岩岩, 王莹, 等. 新疆新源县和四川石渠县啮齿动物及家畜棘球绦虫线粒体cox1基因遗传多态性分析[J]. 中国寄生虫学与寄生虫病杂志, 2020, 38(1):22-29. doi: 10.12140/j.issn.1000-7423.2020.01.004
[22]	Wu CC, Zhang WB, Ran B, et al. Genetic variation of mitochondrial genes among Echinococcus multilocularis isolates collected in western China[J]. Parasit Vectors, 2017, 10(1):265.