Effect of dietary structures on intestinal flora in population infected with Trichuris trichura

doi:10.3969/j.issn.1672-2302.2023.01.007

Abstract

Abstract:

Objective To investigate the effects of different dietary structures on the intestinal flora in population infected by Trichuris trichura (T. trichura) so as to lay a foundation for studying the relationship between intestinal diseases and diet in patients with T. trichura infection. Methods Thirty-four patients infected by T. trichura were recruited from five towns in Lanshan District, Rizhao City, Shandong Province, and their dietary information was collected and analyzed by 16S rDNA high-throughput sequencing to determine the differences in intestinal flora diversity and species composition among T. trichura infected populations with different dietary structures. Results No association was found between high dietary intake and gut flora Alpha diversity or Beta diversity. The three dietary structures of vegetables, dairy products and meat products were significantly associated with the abundance and composition of intestinal flora.There were three species with significant differences in the intake of different vegetables at the genus level. The relative abundance of Clostridium sensu stricto 1 in the PDV3 group with a daily intake of <300 g was higher than that in PDV1 group with a daily intake of >500 g, and the difference was statistically significant (t=2.211, P<0.05). The relative abundance of Ruminococcus in PDV3 group was significantly lower than that in PDV1 group (t=2.246, P<0.05), whereas the relative abundance of Bifidobacterium was lower in PDV3 group than in PDV2 group with daily intake of 300-500 g. The difference was significant (t=2.610, P<0.05). Three species were significantly different in the intake of different dairy products at the genus level. The abundance of Clostridium sensu stricto 1 in PDD2 group with daily intake of 300 g-500 g was higher than that in PDD1 group with daily intake of >500 g(t=3.025, P<0.05), contrarily, the abundance of the Alistipes in PDD3 group with daily intake <300 g was lower than that in PDD1 group (t=3.234, P<0.05). The abundance of Ruminococcaceae UCG-014 was significantly lower in PDD3 group than in PDD2 group (t=2.255, P<0.05). Three species with significant differences in the intake of different meat products at the genus level were observed. The abundance of the genus pair of Phascolarctobacterium and Ruminococcus 2 in PDM2 group with a daily intake of 120-200 g was lower than that in the PDM1 group, with significant difference between groups (t=2.672, P<0.05; t=2.731, P<0.05), yet the relative abundance of Dialister in PDM3 group with daily intake <120 g was lower than that in PDM2 group (t=2.402, P<0.05). Conclusion Different dietary structures affected the composition of intestinal flora in whipworm-infected populations. By supplementing the diet with vegetables, dairy products and meat products to reduce harmful bacteria such as Clostridium sensu stricto 1 and increasing beneficial bacteria such as Bifidobacterium and Alistipes can improve the immunity of the body and reduce or avoid the development of intestinal inflammatory diseases in people with T. trichura infection.

Key words: Trichuris trichura, Dietary structures, Intestinal flora, Relative abundance, Diversity

CLC Number:

R383.1+4

ZHANG Yi-xin, WANG Long-jiang, LIU Jian-cheng, LIU Ping-ping, WANG Yong-bin, XU Yan, YAN Ge, BU Xiu-qin, ZHANG Dian-bo, LI Yue-jin, ZHANG Ben-guang. Effect of dietary structures on intestinal flora in population infected with Trichuris trichura[J]. Journal of Tropical Diseases and Parasitology, 2023, 21(1): 35-43.

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膳食结构	分组	释义
蔬菜	PDV1	每日摄入量>500 g
	PDV2	每日摄入量300~500 g
	PDV3	每日摄入量<300 g
奶制品	PDD1	每日摄入量>500 g
	PDD2	每日摄入量300~500 g
	PDD3	每日摄入量<300 g
肉制品	PDM1	每日摄入量>200 g
	PDM2	每日摄入量120~200 g
	PDM3	每日摄入量<120 g

特征	调查人数	构成比（%）
性别
男	19	55.9
女	15	44.1
年龄（岁）
<40	1	2.9
≥40	33	97.0
职业
农民	32	94.1
工人	1	2.9
学生	1	2.9
文化程度
文盲或半文盲	12	34.3
小学	12	34.3
初中	9	25.7
高中、中专	1	2.9
大学及以上	0	0
蔬菜摄入量（g）
<300	4	11.8
300~500	21	61.8
>500	9	26.5
奶制品摄入量（g）
<300	20	58.8
300~500	10	29.4
>500	4	11.8
肉制品摄入量（g）
<120	10	29.4
120~200	4	11.8
>200	20	58.8

组别	Shannon	Chao1	Observed species	PD whole tree
PDV1	5.77±0.79	744.05±230.86	666.25±203.95	34.16±8.73
PDV2	5.02±1.02	621.38±207.74	555.95±188.33	29.56±8.26
PDV3	5.67±1.24	769.33±262.24	688.75±233.29	35.50±9.87

组别	Shannon	Chao1	Observed species	PD whole tree
PDD1	5.88±0.67	728.90±194.37	659.00±180.85	33.59±8.02
PDD2	5.48±0.89	699.93±197.59	624.70±171.05	32.54±7.24
PDD3	5.05±1.12	640.20±243.75	572.47±220.52	30.33±9.64

组别	Shannon	Chao1	Observed species	PD whole tree
PDM1	5.26±0.98	663.09±212.26	591.39±190.01	31.15±8.38
PDM2	5.15±0.87	606.33±221.33	544.60±200.72	29.31±7.82
PDM3	5.39±1.27	711.16±252.53	639.20±227.12	32.87±10.04