热带病与寄生虫学

热带病与寄生虫学 ›› 2020, Vol. 18 ›› Issue (1): 25-28.

• 论著 • 上一篇    下一篇

白藜芦醇对大肠杆菌诱导的大鼠膀胱粘膜损伤保护作用机制的研究

钱余1 , 蔡茹2∗, 周恩谱1 , 黄进宝1 , 贡东卫1   

  1. 1. 上海市控江医院泌尿外科,上海 200093; 2. 安徽理工大学医学院
  • 收稿日期:2019-12-20 出版日期:2020-03-10 发布日期:2020-03-10
  • 通信作者: ∗通讯作者,蔡茹,E-mail: lxhcrlh@aliyun.com
  • 作者简介:钱余,男,本科,主任医师,研究方向:泌尿外科腔内微创手术治疗。 E-mail:13916216929@139.com
  • 基金资助:
    上海市卫生和计划生育委员会科研课题项目(201740244)

Protective effects of resveratrol on injury of bladder mucosa induced by Escherichia coli in rats

QIAN Yu1 , CAI Ru2∗ , ZHOU En-pu1 , HUANG Jin-bao1 , GONG Dong-wei1   

  1. 1. Department of Urology, Kongjiang Hospital of Shanghai City, Shanghai 200093, China; 2. Department of Microbiology and Para- sitology, Medical School, Anhui University of Science and Technology
  • Received:2019-12-20 Online:2020-03-10 Published:2020-03-10
  • Contact: ∗ Corresponding author, E-mail:lxhcrlh@aliyun.com

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摘要： 目的 探讨白藜芦醇对大肠杆菌诱导的大鼠膀胱粘膜损伤的保护作用机制。 方法 将 45 只(170±10) g 雌性 SD 大鼠随机分为空白对照组、模型对照组和白藜芦醇实验组。 通过尿道向大鼠膀胱内植入导管并进行大肠杆 菌液灌注建立感染性膀胱粘膜损伤模型(模型对照组),白藜芦醇实验组按照 40 mg / kg 剂量灌胃给药,实验结束后 镜检观察大鼠膀胱内壁病理变化。 采用比色法检测超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧 化氢酶(CAT)、一氧化氮(NO)和丙二醛(MDA)含量;采用 ELISA 法检测膀胱组织内细胞因子白介素-1β( IL-1β)、 IL-6、IL-8、IL-10 和肿瘤坏死因子(TNF-α)的含量;采用 Western Blot 法检测转化生长因子-β1(TGF-β1)的表达。 结果 白藜芦醇给药显著减少膀胱黏膜中炎性细胞数量,显著降低大鼠膀胱组织中 MDA、NO 含量,提高了 SOD、GSH -Px、CAT 活性;明显降低 IL -1β、IL-8、TNF-α,提高 IL-6、IL-10 含量;显著减少 TGF-β1 的蛋白表达。 结论 白藜 芦醇通过抗氧化、抗炎和降低纤维化作用改善大肠杆菌诱导的大鼠膀胱粘膜损伤,避免对膀胱组织伤害。

关键词: 白藜芦醇, 膀胱粘膜, 大肠杆菌, 氧化损伤, 细胞因子, 大鼠模型

Abstract: Objective To investigate protective effects of resveratrol on the injury of bladder mucosa induced by Escherichia coli(E. coli) in rats. Methods Forty-five female SD rats, weighting (170±10) g were randomly divided into control group, model group and resveratrol treatment group. Rat models of injury of bladder muscosa were developed E. coli perfusion through the implanted catheter in the urethra, and rats in experimental group were ingragastrically administered with resveratrol in dose of 40 mg / kg. By the end of experiment, bladder mucosae were obtained and microscopically examined for the pathological changes, and colorimetry was used to measure the activities of super oxide dismutase ( SOD), glutathione peroxidase (GSH-Px) and catalase (CAT), malondialdehyde (MDA) and nitric oxide (NO). ELISA was performed to determine the level of interleukin-1β (IL-1β), IL-6, IL-8 and IL-10 as well as tumor necrosis factor-α (TNF-α) in the bladder homogenate, and Western blot was used to measure TGF-β1 expression. Results Resveratrol obviously resulted in decreased inflammatory cell count, MDA and NO level, increased activities of SOD, GSH-Px and CAT, down-regulated TGF-β1 expression, and elevated IL-6 and IL-10 level in the bladder mucosae. Conclusion Resveratrol can improve injury of bladder mucosa in rats induced by E. coli through enhanced anti-inflammatory and anti-oxidative effects, and reducing the fibrosis of the bladder mucosa.

Key words:  Resveratrol, Bladder mucosa, E. coli, Oxidative damage, Cytokines, Rat model

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