热带病与寄生虫学

热带病与寄生虫学 ›› 2014, Vol. 12 ›› Issue (3): 152-.doi: 10.3969/j.issn.1672-2302.2014.03.009

• 论著 • 上一篇    下一篇

快速诊断试剂盒在疟疾诊断中的应用效果

张勇,宋维胜,张西才,张金何   

  1. 236000 安徽阜阳市，阜阳市疾病预防控制中心（张勇），颍上县疾病预防控制中心（宋维胜），太和县疾病预防控制中心（张西才），临泉县疾病预防控制中心（张金何）
  • 出版日期:2014-09-10 发布日期:2014-10-17

The application of rapid diagnostic test in the diagnosis of malaria

Zhang Yong1, Song Weisheng2, Zhang Xicai3, Zhang Jinhe4   

  1. 1. Fuyang Municipal Center for Disease Control and Prevention, Fuyang 23600, China. 2.Yingshang County Center for Disease Control and Prevention. 3. Taihe County Center for Disease Control and Prevention. 4. Linquan County Center for Disease Control and Prevention
  • Online:2014-09-10 Published:2014-10-17

被引次数

11

摘要： 目的 探讨疟疾快速诊断试剂盒临床应用效果，为临床应用提供参考。方法 快速诊断试剂盒与镜检法对103 例疟疾、疑似疟疾进行检测对比分析。结果 镜检疟原虫阳性23 例（Pf 11 例、Pv10 例、Pm 和Po 各1 例），阳性率22.33%；快速诊断试剂盒检测疟原虫阳性22 例（Pf 11 例、Pv 10 例、Pm 1例），阳性率21.36%，卵形疟未检出。两方法检出阳性率无统计学意义（χ2=0.33，P>0.05）。该试剂的总灵敏性95.65% ，总特异性97.50% ，阳性预测值91.67% ，阴性预测值98.73% ，漏检率4.35% ，误诊率2.50%。结论 疟疾快速诊断试剂盒具有良好的疟疾诊断效果和简便、快速等优点，非常适合镜检技术和条件不足的基层医疗、预防机构开展疟疾检测。

关键词: 疟疾, 快速诊断试剂盒, 镜检法

Abstract:

Objective To probe the effect of rapid diagnostic test in the diagnosis of malaria, and provide reference for clinical application. Methods 103 suspicious cases of malaria were checked with rapid diagnostic test and microscopy method. Results 23 positive cases were detected by microscopy method (Pf 11 cases, Pv 10 cases, Pm and Po 1 case each) with positive rate of 22.33%, and 22 positive cases were detected by rapid diagnostic test (Pf 11 cases, Pv 10 cases, Pm 1 case) with positive rate of 21.36%. One Po case was not detected by rapid diagnostic test. There was no significant difference between the two methods (χ2=0.33, P>0.05). The sensitivity of the rapid diagnostic test was 95.65%, with the specificity of 97.5%, the positive predictive value of 91.67%, the negative predictive value of 98.73%, the missing rate of 4.35%, and the misdiagnosis rate of 2.50%. Conclusion The rapid diagnostic test had good effect on the diagnosis of malaria with advantage of simple and fast, and very suitable for grass-roots level medical treatment and disease prevention units which have insufficient microscopy equipment and techniques to carry out the diagnosis of malaria.

Key words: Malaria, Rapid diagnostic test, Microscopy method