热带病与寄生虫学

热带病与寄生虫学 ›› 2014, Vol. 12 ›› Issue (2): 67-69,72.doi: 10.3969/j.issn.1672-2302.2014.02.002

• 论著 • 上一篇    下一篇

3 种青蒿素衍生物对日本血吸虫吡喹酮抗性株童虫的体内作用效果观察

卢萍,汪伟*,曲国立,戴建荣,梁幼生   

  1. 214064 无锡市，江苏省血吸虫病防治研究所、卫生部寄生虫病预防与控制技术重点实验室;*通讯作者
  • 出版日期:2014-06-10 发布日期:2014-08-13
  • 基金资助:

    国家科技支撑计划（2009BAI78B06）；江苏省血地寄防应用性科研课题（X200911、X201111）

In- vivo efficacy of three artesiminin derivatives against schistosomulum of praziquantel- resistant Schistosoma japonicum

Lu Ping, Wang Wei*, Qu Guoli, Dai Jianrong, Liang Yousheng.   

  1. Jiangsu Institute of Parasitic Diseases; Key Laboratory on Technology for Parasitic Disease Prevention and Control, Ministry of Health, Wuxi 214064, China. *Corresponding author.
  • Online:2014-06-10 Published:2014-08-13

被引次数

7

摘要： 目的 观察3 种青蒿素衍生物双氢青蒿素、青蒿琥酯和蒿甲醚对日本血吸虫吡喹酮抗性株童虫的体内作用效果。方法 以经11 轮亚治疗剂量吡喹酮筛选的日本血吸虫为吡喹酮抗性株，以未暴露于吡喹酮的日本血吸虫作为吡喹酮敏感株，收集2 虫株尾蚴感染小鼠，以300 mg/kg 双氢青蒿素、青蒿琥酯和蒿甲醚对感染后7~8 d 童虫分别进行2 次灌服用药（总剂量600 mg/kg），所有小鼠于感染后45 d 解剖，收集小鼠体内成虫并计数，计算减虫率和减雌率。结果 300 mg/kg 双氢青蒿素、蒿甲醚和青蒿琥酯2 日疗法（总剂量600 mg/kg）对日本血吸虫吡喹酮敏感株7~8 d 童虫的减虫率为69.8%~71.0%，减雌率为75.4%~79.8%；对日本血吸虫吡喹酮抗性株7~8 d 童虫的减虫率为64.6%~66.1%，减雌率为69.3%~71.1%，差异均无统计学意义（均P > 0.05）。结论 日本血吸虫吡喹酮抗性株对青蒿素类衍生物双氢青蒿素、青蒿琥酯和蒿甲醚依然敏感，青蒿素衍生物与吡喹酮在日本血吸虫中不存在交叉抗药性。

关键词: 日本血吸虫, 吡喹酮, 青蒿素, 双氢青蒿素, 青蒿琥酯, 蒿甲醚, 交叉抗药性

Abstract:

Objective To evaluate the in- vivo efficacy of three artemisinin derivatives artemether,artesunate and dihydroartemisinin against schistosomulum of praziquantel- resistant Schistosoma japonicum,.Methods The S. japonicum subjected to subcurative dose of praziquantel for 11 rounds was served as a praziquantel- resistant strain, while the field- derived strain without exposure to praziquantel was served as a praziquantel-susceptible strain. The mice infected with the two strains of cercaria were treated with artemether,artesunate and dihydroartemisinin at a single dose of 300 mg/kg by gavage in days 7 and 8 post- infection, respectively. All mice were dissected 45 days post-infection, and the adult worms were collected to estimate worm and female worm burden reductions caused by drug treatment. Results Administration of artemether, artesunate and dihydroartemisinin at a single dose of 300 mg/kg given on days 7 and 8 post-infection achieved total worm burden reductions of 69.8%-71.0% and female worm burden reductions of 75.4%-79.8% in mice infected with praziquantel- susceptible strain, whereas the same treatments reduced total worm burdens of 64.6%-66.1% and female worm burdens of 69.3%-71.1% in mice infected with praziquantel-resistant strain. There were no significant differences between the two strains. Conclusion Praziquantel-resistant S. japonicum remains sensitive to the three major artemisinin derivatives artemether, artesunate and dihydroartemisinin, and no cross-resistance is observed between praziquantel and artemisinin in S. japonicum.

Key words: Schistosoma japonicum, Praziquantel, Artemisinin, Dihydroartemisinin, Artesunate, Artemether, Cross-resistance