热带病与寄生虫学

热带病与寄生虫学 ›› 2024, Vol. 22 ›› Issue (6): 328-333.doi: 10.20199/j.issn.1672-2302.2024.06.002

• 病媒生物防制专题 • 上一篇    下一篇

流行性乙型脑炎病毒阳性蚊虫的病毒多样性研究

闫文喆1,2(), 李晋宇2, 伦辛畅2, 刘蓬勃2, 宋秀平2, 郭玉红2, 刘小波2, 岳玉娟2, 鲁亮2, 赵宁2()   

  1. 1 山东大学齐鲁医学院公共卫生学院媒介生物控制学系，山东济南 250012
    2 传染病溯源预警与智能决策全国重点实验室，中国疾病预防控制中心传染病预防控制所
  • 收稿日期:2024-10-31 出版日期:2024-12-20 发布日期:2025-01-23
  • 通信作者: 赵宁,zhaoning@icdc.cn
  • 作者简介:闫文喆，女，硕士在读，研究方向：媒介生物传染病。E-mail: wzyan0808@163.com
  • 基金资助:
    中国疾病预防控制中心传染病预防控制所技术开发项目(2023-00627)

Study on the virus diversity of the mosquitoes carrying Japanese encephalitis virus

YAN Wenzhe1,2(), LI Jinyu2, LUN Xinchang2, LIU Pengbo2, SONG Xiuping2, GUO Yuhong2, LIU Xiaobo2, YUE Yujuan2, LU Liang2, ZHAO Ning2()   

  1. 1 Department of Vector Control, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan 250012, Shandong Province, China
    2 National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Disease, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention
  • Received:2024-10-31 Online:2024-12-20 Published:2025-01-23
  • Contact: ZHAO Ning,zhaoning@icdc.cn

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摘要：

目的 研究流行性乙型脑炎病毒（Japanese encephalitis virus, JEV）阳性蚊虫携带病毒的病毒组特征，为评估蚊媒病毒的公共卫生风险提供科学依据。方法 根据《全国病媒生物病原学监测方案（试行）》，对全国蚊传病原学监测系统中2022年6—9月成蚊JEV监测结果为阳性的蚊虫样本信息进行收集并开展阳性复核。选择不同省份、不同蚊种中核酸质量高的JEV复核阳性样本进行宏病毒组测序和生物信息分析，比对与拼接组装病毒序列，并对注释到的重要虫媒病毒进行系统发育分析。结果 本研究共选择了贵州、湖北、江苏、重庆、甘肃和云南等6个省份的8个JEV阳性蚊池的蚊虫进行分析，蚊种包括中华按蚊、致倦库蚊和三带喙库蚊。宏病毒组测序结果显示，共注释到41个病毒科和118种已知病毒，其中弹状病毒科（Rhabdoviridae）在半数以上的蚊池中相对丰度较高，有22个病毒科在3种蚊虫中均被注释到。有6个蚊池注释到广平病毒（Quang Binh virus, QBV），拼接组装得到6条QBV近全长序列;有2个蚊池注释到版纳病毒（Banna virus, BAV），拼接组装得到2条BAV第12节段序列;有2个蚊池注释到卡地皮诺病毒（Kadipiro virus, KDV），拼接组装得到1条KDV第1节段序列。系统发育分析显示，本研究的QBV、BAV和KDV病毒株多与近年在我国分离到的病毒株亲缘关系较近，其中2株BAV均为A1基因型。结论 本研究丰富了对中国蚊虫病毒组的认识，揭示了蚊虫中的病毒多样性和QBV、BAV、KDV等虫媒病毒的公共卫生风险，为蚊媒传染病的风险评估和防控提供了重要科学依据。

关键词: 蚊虫, 虫媒病毒, 宏病毒组, 进化分析

Abstract:

Objective To investigate the virome characteristics of the mosquitoes carrying Japanese encephalitis virus (JEV) for evidence in estimating the public health risks arising from mosquito-borne viruses. Methods According to the National Surveillance Program for Vector Pathogens (Trial), the infected mosquito samples carrying JEV were collected in the National Surveillance System for Mosquito-borne Pathogens from June to September 2022, and the data were further verified. Then the JEV positive mosquito samples with high nucleic acid quality from different provinces and distinct mosquito species were selected to undergo virome sequencing and bioinformatics analysis. The viral sequences were aligned and assembled, and phylogenetic analysis was performed in the important arboviruses that were annotated. Results In this study, a total of 8 pools with JEV-positive mosquitoes were investigated from 6 provinces, including Guizhou, Hubei, Jiangsu, Chongqing, Gansu and Yunnan. The mosquito species consisted of Anopheles sinensis, Culex quinquefasciatus and Culex tritaeniorhynchus. The results of virome sequencing showed that a total of 41 virus families and 118 known viruses were annotated. Among them, the abundance of Rhabdoviridae was relatively higher in more than half of the sampling sites. Twenty-two virus families were annotated in all 3 mosquito species. Quang Binh virus (QBV) was annotated in the mosquitoes sampled from 6 pools, and six near-full-length QBV sequences were obtained by assembly. Banna virus (BAV) was annotated in the specimens obtained from two pools, and two segments at the 12th of BAV were obtained by assembly. Two pools were annotated to Kadipiro virus (KDV), and the segment 1st sequence of KDV was obtained by assembly. Phylogenetic analysis showed that most of the QBV, BAV and KDV strains in this study were closely related to the strains isolated in China in recent years, and both BAV strains belonged to genotype A1. Conclusion This study enriched the understanding of mosquito viromes in China, and revealed the diversity of viruses in mosquitoes and the public health risks of arboviruses such as QBV, BAV and KDV. Thus, our findings may be important evidences in assessment of the risks and control of mosquito-borne infectious diseases in scientific fashion.

Key words: Mosquito, Arthropod-borne viruses, Viral metagenomics, Phylogenetic analysis

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