An excerpt of Guideline for Laboratory and Field Trials of Molluscicides for Schistosomiasis Control

doi:10.3969/j.issn.1672-2302.2023.06.001

Abstract

Abstract:

The World Health Organization (WHO) released the Guideline for Laboratory and Field Trials of Molluscicides for Schistosomiasis Control (hereinafter referred to as the "Guideline") in 2019. The Guideline aims to provide a standardized process for the efficacy test and evaluation of molluscicides, so as to provide a basis for national regulatory authorities to conduct product registration and other work. This article translates the background and three stages of laboratory studies, small-scale field trials, and large-scale field trials stipulated in the Guideline for reference.

Key words: Schistosomiasis, Molluscicide, Efficacy test, Guideline, World Health Organization

CLC Number:

R532.21

JIN Wei, ZHANG Guangming, HUANG Yinyin, CAO Zhiguo, WANG Tianping. An excerpt of Guideline for Laboratory and Field Trials of Molluscicides for Schistosomiasis Control[J]. Journal of Tropical Diseases and Parasitology, 2023, 21(6): 305-310.

Figures/Tables 4

Table 1

Figure 1

Table 2

Figure 2

阶段	试验类型	目的
Ⅰ	室内试验	①建立剂量-反应曲线（确定LC₅₀和LC₉₅） ②对成螺及螺卵的毒力（确定有效浓度和残留药效） ③用于抗药性监测的鉴别浓度* ④交叉抗性评估 ⑤人体危害评估^#
Ⅱ	小规模现场试验	①在半野外和有限的自然条件下，观察其对螺类的早期毒性和持续毒性 ②确定最佳的施药方法和剂量
Ⅲ	大规模现场试验	①在大面积天然水体等环境中，观察最佳施药量下对螺类的早期毒性和持续毒性 ②观察运行情况和社会接受情况 ③记录对非靶标生物的影响

试验/对照浓度(mg/mL)	重复试验
试验/对照浓度(mg/mL)	第1次试验	第2次试验	第3次试验
浓度1	第1天	第2天	第3天
浓度2	第1天	第2天	第3天
浓度3	第1天	第2天	第3天
浓度4	第1天	第2天	第3天
浓度5	第1天	第2天	第3天
浓度6	第1天	第2天	第3天
空白对照	第1天	第2天	第3天
经典杀螺剂对照	第1天	第2天	第3天

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