Genetic differentiation of Oncomelania hupehensis by microsatellite and mitochondrial molecular biotechnology

doi:10.20199/j.issn.1672-2302.2024.06.011

Abstract

Abstract:

Oncomelania hupensis is the sole intermediate host of Schistosoma japonicum. Its geographical distribution plays crucial roles in the transmission and prevalence of schistosomiasis. Therefore, investigation on the snail genetic differentiation is greatly significant for snail and schistosomiasis control. With rapid development of molecular biology, a variety of molecular markers and sequence determination techniques have been widely used in the study of genetic differentiation of O. hupensis, among which DNA molecular markers and mitochondrial DNA sequence determination techniques possess obvious superiority in the study of genetic differentiation of O. hupensis population. This paper reviews the genetic differentiation of O. hupensis by using microsatellite DNA markers and mitochondrial DNA sequencing techniques.

Key words: Oncomelania hupensis, Genetic differentiation, Microsatellite DNA, Mitochondria DNA

CLC Number:

R184.38

LI Chunying, CUI Xiao, DU Chunhong, WANG Hongqiong, YANG Zaogai, Wang Yunsong, SONG Jing, DONG Yi. Genetic differentiation of Oncomelania hupehensis by microsatellite and mitochondrial molecular biotechnology[J]. Journal of Tropical Diseases and Parasitology, 2024, 22(6): 375-381.

Figures/Tables 1

Table 1

Basic information of microsatellite loci and their primer sequences

位点	引物序列（5′→3′）	重复单元	位点大小（bp ）	等位基因大小（bp ）	文献出处
（CA）₈RY	CACACACACACA —CACARY				[11-12]
P84	F : GTTGCAGATTCCGAAAGA	(TACAT)₃	140	130-160	[4,14]
P84	R: GATCAGGGGTTGTCCAGT	(TACAT)₃	140	130-160	[4,14]
T5-13	F: TAGTGGGACTTATTTGCTG	(GT)₁₄…(GT)₆	245	193-99	[4,13-14]
T5-13	R: AAGGCTGAGTGGTAGTTA	(GT)₁₄…(GT)₆	245	193-99	[4,13-14]
T5-11	F: ACGCCAGTCTTGGTGTCA	(GT)₁₄	173	157-207	[4,13-14]
T5-11	R: TACTTGGGCAGAAGGGTT	(GT)₁₄	173	157-207	[4,13-14]
T4-22	F: TATCCAAGAAGCCGAAAC	（CA)₁₀	244	232-276	[4,13-14]
T4-22	R: GAGGAAAGCGAGGTAAGA	（CA)₁₀	244	232-276	[4,13-14]
T6-27	F: AATGACACCCCGAACAAA	(G)₁₃…(GT)₆ GG(GT)₁₂	204	164-212	[4,13-14]
T6-27	R: CACTTCTCAACTCCAACCT	(G)₁₃…(GT)₆ GG(GT)₁₂	204	164-212	[4,13-14]
P82	F : AAGAACTGCTCATACTGGAAAG	(GGA)₄(GAA)₁₂ (TCCTA)₂	179	170-239	[4,13-14]
P82	R: GTGGTGCCCCTACGACCT	(GGA)₄(GAA)₁₂ (TCCTA)₂	179	170-239	[4,13-14]
T6-47	F: CCGAAGTGATAGAAACCG	(GT)₆(GT)₉	190		[13]
T6-47	R: AGGCAGAAATGGGCAGAC	(GT)₆(GT)₉	190		[13]
T5-21	F: ATAAGTTTAGCCAGTCACC	(GT)₁₆(GT)₇	199		[13]
T5-21	R: ACACGCAGTCCACGCACA	(GT)₁₆(GT)₇	199		[13]
T4-36	F: CGGGTTACGGGAAAGGAT	(CA)₁₇	252		[13]
T4-36	R: AGGGACGAACTCACGAAG	(CA)₁₇	252		[13]
E3	F: CCAAACCCTCTTCAACAC	(CA)₁₅	190		[13]
E3	R: GCCGAAAGAAATTCTACG	(CA)₁₅	190		[13]
E15	F: AAAGAACCGAATCAGGAC	(CA)₂₁(CA)₁₄ (CA)₈	189		[13]
E15	R: TACCAGCCGATGAATAAA	(CA)₂₁(CA)₁₄ (CA)₈	189		[13]
D17	F: CATCGTTGACACGGGTTT	(GT)₃₂	232		[13]
D17	R: TGGGCTGTTGGTCCTCTT	(GT)₃₂	232		[13]
C23	F: CTGGACCTAAAGCAATAAC	(GT)₁₄	172		[13]
C23	R: GAGCCAATCACCTAAACTA	(GT)₁₄	172		[13]
B14	R: AGAAAGCAGCATGACCCA	(CT)₉	337		[13]
B14	F: AGGTGGCATTATCGAATT	(CT)₉	337		[13]
T6-17	F：GGCCTGCCTTGGTTTTTTCACGTAG	(AC)₈	244		[15-16]
T6-17	R: AGCTTGGGATCATCTCCAGGTC	(AC)₈	244		[15-16]
P101	F: AGGTCACGCTTTCTGCATTT	(TG)₁₃(TTG)₈	181		[15-16]
P101	R: GTGCAACCATGTGTTGTGGT	(TG)₁₃(TTG)₈	181		[15-16]
D11	F: AGCTTGGGATCAGAATGTCGTTTGT	(TG)₁₇	218		[15-16]
D11	R: TATGTAGATGTTCACTGGTTTGTCC	(TG)₁₇	218		[15-16]
B14	F: CAGTCACAGCGCAGCCTACGA	(AG)₃₃	207		[15-16]
B14	R: TCAAGCGACCTGATGTCAAATACC	(AG)₃₃	207		[15-16]
T4-33	F: GTCAAAACAACGAGGGCTGT	(AC)₁₉	171		[15-16]
T4-33	R: CTGAGTGGAATGGGAGTTGG	(AC)₁₉	171		[15-16]
C22	F: TGGGATCGGTACATCTGGATAGTGG	(CA)21	235		[15-16]
C22	R: GGGATCAATGAAAGTTCTTGCGTTC	(CA)21	235		[15-16]
A18	F: GCCGATGATACAAGACCC	(CT)18	139		[17-18]
A18	Rf: GAGAATCTCCAGGCACGC	(CT)18	139		[17-18]
C22	F: CGGTACATCTGGATAGTGG	(CA)21	189		[17-18,20???-24]
C22	Rf: TGCGAAACAGTTGCAGACAC	(CA)21	189		[17-18,20???-24]
T4-33	F: ACGTCAAAACAACGAGGGC	(AC)19	175		[17-18]
T4-33	R: TACTGAGTGGAATGGGAG	(AC)19	175		[17-18]
T6-17	F: GCTGTCCTTTTACCAACTGC	(AC)8	192		[17-18]
T6-17	R: TATCAAAGGATTATGCCGAG	(AC)8	192		[17-18]
D11	F: TTCAGTTGTCTTATTTCGTG	(TG)17	167		[17]
D11	R: TAGATGTTCACTGGTTTGTC	(TG)17	167		[17]
B14	F: GAAGGCGGCAGCAGCAAC	(AG)33	149		[17]
B14	R: AGCGACCTGATGTCAAATAC	(AG)33	149		[17]
T4-43	F: TCGGTTACTAGCAAGACG	(GA)33	242		[17]
T4-43	R: TCGAATGGATAGGAGCAT	(GA)33	242		[17]
E3	F: GATTTGTGAAAGTGAGGGTA	(CA)33	255		[17]
E3	R: TAGCAGGCGTCAAGGTAA	(CA)33	255		[17]
T1-10	F: TCACTCGGGTGTAATGCT	(GA)38	228		[17]
T1-10	R: TTTGTTACTGATGGTGGC	(GA)38	228		[17]
T4-25	F: CAATAGTTCGACTCGGAAGA	(CT)35	180		[17]
T4-25	R: CGAGGTATGGCGTTGCTI	(CT)35	180		[17]
B14	F: GGCAGTTTTGATTAGCTTGGT	(AG)₃₃	172		[20???-24]
B14	R: ACCTGATGTCAAATACCAGTTAG	(AG)₃₃	172		[20???-24]
T6-17	F: GCTGTCCTTTTACCAACTGC	(AC)₈	192		[20???-24]
T6-17	R: TATCAAAGGATTATGCCGAG	(AC)₈	192		[20???-24]
DH02	F: AGGCGATTTATGCAGTTCCC	(AGA)₁₂	218		[20???-24]
DH02	R: TGCGAAACAGTTGCAGACAC	(AGA)₁₂	218		[20???-24]
DH01	F: GATCAATACCAGCAACTACT	(TCA)₃₃	462		[20???-24]
DH01	R: ATGATGCAGACTTGTAACGC	(TCA)₃₃	462		[20???-24]
E3	F: CTTGTGACGATGACGACCCT	(CA)₃₃	302		[21-22]
E3	R: GCGGGTTATGAGCTGCGATTAC	(CA)₃₃	302		[21-22]
C23	F: GTCCGGTCCTGATTTGGCTA	(GT)₁₄	339		[21-22]
C23	R: GGAATGGAGCGAGGTTCTTAC	(GT)₁₄	339		[21-22]
D17	F: TTTAGTCCCCTTTGGCGTGTG	(GT)₃₂	238		[21-22]
D17	R: GCTTGGCCTCGTTACCCAATAT	(GT)₃₂	238		[21-22]
T4-36	F: CGGGTTACGGGAAAGGAT	(CA)₁₇	248		[21-22]
T4-36	R: AGGGACGAACTCACGAAG	(CA)₁₇	248		[21-22]
T4-33	F: GTCAAAACAACGAGGGCTGT	(AC)19	171		[21-22]
T4-33	R: CTGAGTGGAATGGGAGTTGG	(AC)19	171		[21-22]
T1-10	F: TCACTCGGGTGTAATGCT	(GA)₃₈		173-259	[20,23-24]
T1-10	R: TTTGTTACTGATGGTGGC	(GA)₃₈		173-259	[20,23-24]
T4-25	F: CAATAGTTCGACTCGGAAGA	(CT)₃₅		142-228	[20,23-24]
T4-25	R: CGAGGTATGGCGTTGCTI	(CT)₃₅		142-228	[20,23-24]
T4-22	F: TATCCAAGAAGCCGAAAC	（CA)₁₀		224-256	[20,23-24]
T4-22	R: GAGGAAAGCGAGGTAAGA	（CA)₁₀		224-256	[20,23-24]
D11	F: CAGAATGTCGTTTGTGGTGGT	(TG)₁₇	206		[21-22,24]
D11	R: TGTAGATGTTCACTGGTTTGTC	(TG)₁₇	206		[21-22,24]

Table 1

References 49

[1]	周晓农. 实用钉螺学[M]. 北京: 科学出版社, 2005.
[2]	Utzinger J, Keiser J. Schistosomiasis and soil-transmitted helminthiasis: common drugs for treatment and control[J]. Expert Opin Pharmacother, 2004, 5(2):263-285.
[3]	沈美芬, 杜春红, 宋静, 等. 云南省2021年血吸虫病风险监测[J]. 中国热带医学, 2023, 23(2):157-161. doi: 10.13604/j.cnki.46-1064/r.2023.02.10
[4]	李石柱. 应用现代生物信息技术对湖北钉螺遗传多样性的研究[D]. 北京: 中国疾病预防控制中心, 2009.
[5]	李石柱, 王强, 钱颖骏, 等. 中国大陆湖北钉螺种下分化研究进展[J]. 中国血吸虫病防治杂志, 2009, 21(2):150-153.
[6]	周延清. 遗传标记的发展[J]. 生物学通报, 2000, 35(5):17-18.
[7]	操治国, 赵亚娥, 汪天平. 湖北钉螺分类鉴定技术研究进展[J]. 中国血吸虫病防治杂志, 2015, 27(1):98-103.
[8]	王艺秀. 湖北钉螺(Oncomelania hupensis)微卫星DNA文库的建立及多态性位点的筛选[D]. 西安: 陕西师范大学, 2009.
[9]	Jones CS, Lockyer AE, Rollinson D, et al. Isolation and characterization of microsatellite loci in the freshwater gastropod, Biomphalaria glabrata, an intermediate host for Schistosoma mansoni[J]. Mol Ecol, 1999, 8(12):2149-2151. pmid: 10632870
[10]	Viard F, Bremond P, Labbo R, et al. Microsatellites and the genetics of highly selfing populations in the freshwater snail Bulinus truncatus[J]. Genetics, 1996, 142(4):1237-1247. doi: 10.1093/genetics/142.4.1237 pmid: 8846901
[11]	牛安欧, 熊衍文. 微卫星锚定PCR研究湖北钉螺的遗传变异[J]. 中国寄生虫病防治杂志, 2002(4):42-45.
[12]	周艺彪, 赵根明, 韦建国, 等. 微卫星锚定PCR分析19个湖北钉螺种群之间的遗传变异关系[J]. 中华流行病学杂志, 2007, 28(9):859-862.
[13]	李石柱, 王艺秀, 马雅军, 等. 湖北钉螺多态微卫星DNA位点筛选和特征初步分析[J]. 中国血吸虫病防治杂志, 2010, 22(2):122-125.
[14]	李石柱, 张丽, 刘琴, 等. 应用微卫星DNA标记探讨我国长江中下游地区湖北钉螺群体遗传结构[J]. 中国寄生虫学与寄生虫病杂志, 2012, 30(4):268-273.
[15]	崔斌. 长江中下游地区湖北钉螺小尺度景观群体的种群遗传结构研究[D]. 西安: 陕西师范大学, 2014.
[16]	崔斌, 官威, 尤平, 等. 基于微卫星标记的不同壳型湖北钉螺小尺度景观群体遗传结构研究[J]. 中国人兽共患病学报, 2014, 30(7):701-708.
[17]	何健. 基于时空与遗传信息的江苏省江滩钉螺分布规律研究[D]. 无锡: 江苏省血吸虫病防治研究所, 2016.
[18]	何健, 缪峰, 杨坤, 等. 基于微卫星DNA标记的山东微山湖区放养钉螺遗传结构分析[J]. 中国寄生虫学与寄生虫病杂志, 2016, 34(3):261-265.
[19]	顾伟玲, 彭晗琪, 张韩香, 等. 2022年浙江省嘉兴市湖北钉螺的群体遗传学分析[J]. 上海预防医学, 2024, 36(6):559-562,569.
[20]	邹惠莹. 基于微卫星位点和线粒体COX1基因对江苏省苏州市部分地区湖北钉螺遗传多样性的研究[D]. 苏州: 苏州大学, 2021.
[21]	丁焕. 应用微卫星位点研究安徽、江苏地区湖北钉螺的群体遗传结构[D]. 苏州: 苏州大学, 2018.
[22]	周支军, 沈璐, 华雪涛, 等. 基于微卫星标志构建湖北钉螺PCR多重反应体系[J]. 寄生虫病与感染性疾病, 2021, 19(3):163-167.
[23]	邓姚. 基于微卫星(STR)位点的新发现、复现和现有有螺环境中钉螺遗传多样性分析[D]. 苏州: 苏州大学, 2019.
[24]	Qiu C, Lu DB, Deng Y, et al. Population genetics of Oncomelania hupensis snails, intermediate hosts of Schistosoma japonium, from emerging,re-emerging or established habitats within China[J]. Acta Trop, 2019, 197:105048.
[25]	Cheng YH, Sun MT, Wang N, et al. Population Genetics of Oncomelania hupensis Snails from New-Emerging Snail Habitats in a Currently Schistosoma japonicum Non-Endemic Area[J]. Trop Med Infect Dis, 2023, 8(1):42.
[26]	Song J, Wang HQ, Li SZ, et al. The genetic diversity of Oncomelania hupensis robertsoni,intermediate hosts of Schistosoma japonicum in hilly regions of China, using microsatellite markers[J]. Parasit Vectors, 2024, 17(1):147.
[27]	李石柱, 王艺秀, 刘琴, 等. 湖北钉螺线粒体基因组全序列测定研究[J]. 中国寄生虫学与寄生虫病杂志, 2009, 27(4):291-296.
[28]	韩庆霞, 牛安欧, 李金木. 不同地域株湖北钉螺CO1基因的差异研究[J]. 中国人兽共患病杂志, 2005, 21(4):320-322.
[29]	张仪, Yamasaki Hiroshi, 刘和香, 等. 子一代实验室钉螺细胞色素c氧化酶Ⅰ、细胞色素b基因序列分析[J]. 中国寄生虫学与寄生虫病杂志, 2004, 22(5):274-276.
[30]	Wilke T, Davis GM, Chen CE, et al. Oncomelania hupensis (Gastropoda: rissooidea) in Eastern China: molecular phylogeny, population structure, and ecology[J]. Acta Trop, 2000, 77(2):215-227. pmid: 11080513
[31]	石朝辉, 邱持平, 夏明仪, 等. 湖北省庙河地区钉螺细胞色素C氧化酶1基因差异的研究[J]. 中国寄生虫学与寄生虫病杂志, 2001, 19(1):41-44.
[32]	白洁. 云南省血吸虫病流行区湖北钉螺COI基因序列分析[D]. 昆明: 昆明医学院, 2008.
[33]	倪凤娥, 杨鹏华, 黄敏. 湖北钉螺CO1基因差异及系统进化[J]. 湖北农业科学, 2009, 48(9):2069-2070,2073.
[34]	陈琳, 张锡林, 何谐, 等. 三峡库区上、下游血吸虫病流行区钉螺线粒体cox1基因遗传变异研究[J]. 热带医学杂志, 2010, 10(1):5-8,4.
[35]	杨芳芳. 广西肋壳钉螺和光壳钉螺与邻省钉螺亲缘关系研究[D]. 南宁: 广西医科大学, 2011.
[36]	李洪军, 汪伟, 张伟, 等. 长江中下游地区湖北钉螺线粒体COⅠ基因遗传变异研究[J]. 海洋科学, 2011, 35(10):86-90.
[37]	Davis G, Wilke T, Zhang Y, et al. Snail-Schistosoma, Paragonimus interactions in China: population ecology, genetic diversity, coevolution and emerging diseases[J]. Malacologia, 1999, 41:355-377.
[38]	陈申, 段磊, 李胜明, 等. 常德市钉螺种群生态隔离机制的初步探析[J]. 中国血吸虫病防治杂志, 2023, 35(2):147-154.
[39]	林睿, 黎学铭, 胡缨, 等. 广西、湖南、云南三地钉螺Cytb基因序列变异和系统进化研究[J]. 中国病原生物学杂志, 2008, 3(2):110-113.
[40]	Spolsky CM, Davis GM, Yi Z. Sequencing methodology and phylogenetic analysis: cytochrome b gene sequence reveals significant diversity in Chinese populations of Oncomelania (Gastropoda: Pomatiopsidae)[J]. Malacologia, 1996, 38(1/2):213-221.
[41]	胡缨, 黎学铭, 林睿, 等. 三地钉螺线粒体DNA两个分子的遗传变异研究[J]. 中国寄生虫学与寄生虫病杂志, 2007, 25(6):474-477.
[42]	蒋奉娟, 刘卉萌, 陈海婴, 等. 湖北钉螺指名亚种一输入性扩散事件的群体遗传学[J]. 南昌大学学报(理科版), 2020, 44(3):286-294,299.
[43]	Pruess KP, Adams BJ, Parsons TJ, et al. Utility of the mitochondrial cytochrome oxidase II gene for resolving relationships among black flies (Diptera: Simuliidae)[J]. Mol Phylogenet Evol, 2000, 16(2):286-295. pmid: 10942615
[44]	Moulton JK. Can the current molecular arsenal adequately track rapid divergence events within Simuliidae (Diptera)?[J]. Mol Phylogenet Evol, 2003, 27(1):45-57. pmid: 12679070
[45]	Li SZ, Zhang L, Ma L, et al. Phylogenetic performance of mitochondrial protein-coding genes of Oncomelania hupensis in resolving relationships between landscape populations[J]. J Sytematics Evolution, 2013, 51(3):353-364.
[46]	贾悦, 戴建荣. 湖北钉螺分类研究进展[J]. 中国血吸虫病防治杂志, 2012, 24(4):486-490.
[47]	王晨阳, 王璐, 张锐虎, 等. SNP标记在动物遗传育种及人类疾病动物模型研究中的应用[J]. 中国比较医学杂志, 2019, 29(4):120-125.
[48]	马敏彪, 武利恒, 丁琳, 等. 基于SNP分子标记的湖羊群体遗传结构分析[J]. 畜牧与兽医, 2024, 56(6):1-8.
[49]	Dementieva NV, Shcherbakov YS, Tyshchenko VI, et al. Comparative analysis of molecular RFLP and SNP markers in assessing and understanding the genetic diversity of various chicken breeds[J]. Genes, 2022, 13(10):1876.