热带病与寄生虫学

热带病与寄生虫学 ›› 2021, Vol. 19 ›› Issue (4): 236-.

• 综述 • 上一篇    

变应原体外检测技术研究进展

周小倩1,井刚1,王睿怡1,张文远1,王文静1,唐小牛2,孙恩涛1
  

  1. 1. 皖南医学院卫生检验与检疫学教研室,安徽 芜湖 241002; 2. 皖南医学院医学寄生虫学教研室
  • 出版日期:2021-08-20 发布日期:2021-08-26
  • 通信作者: 唐小牛,E-mail:txniu@ 163. com;孙恩涛,E-mail:asdentao@ 126. com
  • 作者简介:周小倩,女,硕士在读,研究方向:病原生物学。 E-mail:861514965@ qq. com
  • 基金资助:
    国家自然科学基金项目(31870352);皖南医学院 2019 年青年卓越人才资助项目( wyqnyx201902);安徽省省级大学生创新创业项目
    (S201910368110)

Advances in detection of allergens in vitro technique

ZHOU Xiao-qian1,JING Gang1,WANG Rui-yi1,ZHANG Wen-yuan1,WANG Wen-jing1,TANG Xiao-niu2,SUN En-tao1   

  1. 1. Department of Health Inspection and Quarantine,Wannan Medical College,Wuhu 241002,Anhui Province,China;
    2    . Department of Medical Parasitology,Wannan Medical College
  • Online:2021-08-20 Published:2021-08-26

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摘要： 变应原的准确检测对过敏性疾病的预防和诊疗至关重要。 体外检测是当前变应原检测的主要研究方向。 本文主要对检测血清 sIgE 的放射变应原吸附试验、酶联免疫吸附试验、荧光酶免疫分析、纳米磁微粒化学发光法、免疫印迹法、液相芯片法,以及直接检测变应原的生物共振法进行了概述,并对各种体外检测技术的优缺点进行了比较,以期为临床上选择合适的变应原体外检测方法提供参考。

关键词: 变应原, 特异性 IgE, 体外检测

Abstract: Accurate detection of allergens is vital for the prevention,diagnosis and treatment of allergic conditions. Currently, in vitrotechnique represents the leading research interest in allergen detection. This paper summarizes the available detection techniques targeting at serum sIgE measurement,including radioallergosorbent test,enzyme-linked immunosorbent assay,fluoroenzyme immunoassays,magnetic nano-particle chemiluminescence immunoassay,immunoblotting assay,liquidphase chip method as well as bioresonance method for direct detection of the allergens. In addition,the advantages and disadvantages are also compared pertinent to various in vitro detection techniques,with an attempt to provide reference for clinical option in selection of appropriate in vitrotechnique in detecting allergen in specific subjects.

Key words: Allergen, specific IgE, In vitro detection

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