热带病与寄生虫学

热带病与寄生虫学 ›› 2020, Vol. 18 ›› Issue (4): 239-242.

• 综述 • 上一篇    下一篇

核酸检测技术在棘球蚴病诊断中的应用研究进展

王奇1,贺平1∗,万里2∗
  

  1. 1. 西藏民族大学医学部,咸阳 712082; 2. 西藏自治区第二人民医院外一科
  • 出版日期:2020-11-27 发布日期:2020-11-27
  • 作者简介:王奇,女,硕士研究生,研究方向:寄生虫感染与免疫。 E-mail:675648298@qq.com ∗通讯作者,贺平,E-mail:hepingyixue@126.com;万里,E-mail:496791784@qq.com
  • 基金资助:
    国家自然科学基金(81860365);西藏自治区自然科学基金( XZ2018ZRG-80( Z));西藏民族大学重大项目培育计划(18MDZ02);西藏民族大学教学改革项目(2017358)

Research progress in nucleic acid detection in the diagnosis of echinococcosis

WANG Qi1, HE Ping1∗, WAN Li2∗   

  1. 1. Medical School of Xizang Minzu University, Xianyang 712082, China; 2. No. 1 Surgery Department, The Second People's Hospital of Tibet Autonomous Region
  • Online:2020-11-27 Published:2020-11-27

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摘要： 棘球蚴病是一种严重危害公共健康的人兽共患寄生虫病。 由于影像学和免疫学诊断存在一定局限性,近年来国内外学者针对病原体棘球绦虫建立了多种核酸检测方法,主要包括聚合酶链式反应(polymerase chain reaction, PCR)、多重引物 PCR(multiple-PCR)、实时荧光定量 PCR(qRT-PCR)、环介导等温扩增核酸技术( LAMP)等,本文对这些方法的应用研究进行了归纳,探讨这些检测方法在棘球蚴病诊断中意义。

关键词: 棘球蚴病, 核酸检测, 细粒棘球绦虫, 多房棘球绦虫

Abstract: Echinococcosis is one kind of zoonotic parasitic disease that seriously endangers public health. In view of the limitations of imaging and immunological diagnosis, many nucleic acid techniques have been developed for detecting the pathogenic echinococcus in recent years, including polymerase chain reaction(PCR), multiple-PCR, real-time fluorescence quantitative PCR(qRT-PCR) and loop-mediated isothermal amplification(LAMP). This paper summarized the progress of application of nucleic acid technique to the diagnosis of echinococcosis, together with the application value being investigated.

Key words: Echinococcosis, Nucleic acid detection, Echinococcus granulosus, Echinococcus multilocularis

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