环介导等温扩增技术在寄生虫检测中的应用进展

热带病与寄生虫学 ›› 2020, Vol. 18 ›› Issue (4): 234-238,233.

环介导等温扩增技术在寄生虫检测中的应用进展

朱志伟,赵金红^∗

皖南医学院医学寄生虫学教研室,芜湖 241002

出版日期:2020-11-27 发布日期:2020-11-27
作者简介:朱志伟,男,硕士,研究方向:病原生物的生态学与流行病学。 E-mail:497582734@qq.com ∗通讯作者,赵金红,E-mail: jhjhzhao@aliyun.com
基金资助:
安徽省自然科学基金面上项目(1608085MC77);安徽省高校优秀青年人才支持计划重点项目( gxyqZD2016171);安徽省高校学科(专业)拔尖人才学术资助项目(gxbjZD2020071)

Application progress of loop-mediated isothermal amplification technology in the diagnosis of human parasitic diseases

ZHU Zhi-wei, ZHAO Jin-hong^∗

Department of Medical Parasitology, Wannan Medical College, Wuhu 241000, China

Online:2020-11-27 Published:2020-11-27

摘要/Abstract

摘要： 环介导等温扩增(Loop-mediated isothermal amplification, LAMP)技术是近年来发展起来的一种分子生物学检测方法,具有简便快捷、敏感特异、实用等优点,并已在人体和动物寄生虫检测领域中得到广泛应用,本文主要就 LAMP 技术在原虫、吸虫、线虫、绦虫等寄生虫检测中的应用进行介绍。

关键词: 环介导等温扩增技术, 寄生虫病, 实验室检测, 研究进展

Abstract: Loop-mediated isothermal amplification (LAMP) is a molecular biology diagnostic technology developed in the past few years, with simplicity, sensitivity, specificity and practicability, and has been widely used in the diagnosis of human and animal parasitic diseases. This article aims at describing the application of LAMP technology to detecting protozoa, nematodes, flukes, tapeworm infections.

Key words: Loop-mediated isothermal amplification, Parasitic diseases, Laboratory diagnosis, Research progress

中图分类号:

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朱志伟, 赵金红. 环介导等温扩增技术在寄生虫检测中的应用进展[J]. 热带病与寄生虫学, 2020, 18(4): 234-238,233.

ZHU Zhi-wei, ZHAO Jin-hong. Application progress of loop-mediated isothermal amplification technology in the diagnosis of human parasitic diseases[J]. Journal of Tropical Diseases and Parasitology, 2020, 18(4): 234-238,233.