热带病与寄生虫学

热带病与寄生虫学 ›› 2020, Vol. 18 ›› Issue (1): 17-20.

• 论著 • 上一篇    下一篇

低温胁迫下淡色库蚊体内蛋白表达的双向电泳分析

马荣1,2 , 刘丽娟1∗   

  1. 1. 山东省寄生虫病防治研究所,山东第一医科大学(山东省医学科学院),济宁 272033; 2. 山东省济宁市第一人民医院
  • 收稿日期:2020-01-25 出版日期:2020-03-10 发布日期:2020-03-10
  • 通信作者: ∗通讯作者,刘丽娟,E-mail:jj8liu@sina.com
  • 作者简介:马荣,女,硕士,主管检验师。 研究方向:病原菌耐药机制。 E-mail:marong8902@126.com
  • 基金资助:
    国家自然基金(81902096);山东省医学科学院医药卫生科技创新工程项目

Analysis of low temperature stress induced proteomic changes in Culex pipiens pallens via two-dimensional electrophoresis

Ma Rong1,2 , Liu Li-juan1∗   

  1. 1. Shandong Institute of Parasitic Diseases, Shandong First Medical University,Shandong Academy of Medical Sciences, Jining 272033, Shandong, China; 2. Jining No. 1 People'Hospital
  • Received:2020-01-25 Online:2020-03-10 Published:2020-03-10
  • Contact: ∗ Corresponding author,E-mail:jj8liu@sina.com

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摘要： 目的 探索低温胁迫对淡色库蚊体内蛋白质表达的影响。 方法 以室内饲养淡色库蚊为对照,利用双向 电泳技术,比较低温处理后淡色库蚊体内蛋白质表达谱的差异。 结果 处理组和对照组蛋白质浓度分别为 8. 5 μg / μL 和 4. 76 μg / μL,二者差异有统计学意义(P<0. 05)。 经双向电泳分析,共发现 2 倍差异蛋白点 77 个,低温处理组 表达量上调的蛋白点 30 个,表达量下调的蛋白点 47 个;低温处理组特有蛋白点 14 个,分子量主要在 25 ~ 60 kDa 之 间。 共鉴定出 25 个差异表达蛋白质,主要与细胞骨架的重塑、糖代谢、能量代谢以及各种合成酶和代谢酶有关。 结论 低温处理可诱导淡色库蚊体内蛋白质的表达变化,对差异蛋白的进一步分析有望获得淡色库蚊的抗冻蛋白。

关键词: 淡色库蚊, 低温胁迫, 双向电泳, 质谱分析

Abstract: Objective To explore the effects of low temperature stress on protein expression in Culex pipiens pallens. Methods Two-dimensional electrophoresis was performed to compare the differences in protein expression profiles of Cx. pipiens pallens after low temperature treatment using indoor bred Cx. pipiens pallens as control. Results The protein concentration was 8. 5 μg / μL and 4. 76 μg / uL respectively for the treatment group and the control group, and the difference was significant (P<0. 05). Two-dimensional electrophoresis analysis revealed a total of 77 2-fold differential protein spots, in which up-regulated expression was seen in 30 protein spots, and down-regulation was observed in another 47 protein spots in low temperature treatment group. Fourteen unique protein spots were detected in the low temperature treatment group, with molecular weight ranging from 25 to 60 kDa. A total of 25 differentially expressed proteins were identified, mainly re- lated to cytoskeleton remodeling, carbohydrate metabolism, energy metabolism, and various synthases and metabolic en- zymes. Conclusion Low temperature treatment can induce changes in protein expression in Cx. pipiens pallens. Further analysis of the differential proteins may be promising to obtain antifreeze proteins from Cx. pipiens pallens.

Key words: Culex pipiens pallens Coquillett, Low temperaturestress, Two-dimensional electrophoresis, Mass spectrometry