热带病与寄生虫学

热带病与寄生虫学 ›› 2014, Vol. 12 ›› Issue (3): 169-.doi: 10.3969/j.issn.1672-2302.2014.03.015

• 论著 • 上一篇    下一篇

溶血磷脂酰胆碱对人脐静脉内皮细胞血管紧张素系统的影响

戴文卓,孔岳南* ,王鹏   

  1. 214001 江苏无锡市，南京医科大学附属无锡市第二人民医院神经内科（戴文卓、孔岳南），南京医科大学附属无锡市第二人民医院神经外科（王鹏）
  • 出版日期:2014-09-10 发布日期:2014-10-17

The effect of Lysophosphatidyl choline on RAS of human umbilical vein endothelial cells

Dai Wenzhuo, Kong Yuenan*, Wang Peng   

  1. Department of Neurosurgery, Affiliated The Second People's Hospital of Wuxi City, Nanjing Medical University, Wuxi 214002, China
  • Online:2014-09-10 Published:2014-10-17

摘要： 目的 观察溶血磷脂酰胆碱（Lysophosphatidyl choline，LPC）对人脐静脉内皮细胞（Human umbilical vein endothelial cells，HUVECs）血管紧张素系统的影响。方法 体外培养HUVECs，分为正常对照组、低浓度（10μg/L）LPC 组、中浓度（20μg/L）LPC 组和高浓度（40 μg/L）LPC 组。采用放射免疫法和RT-PCR 法检测LPC 对HUVECs 血管紧张素Ⅱ（AngiotensinⅡ，AngⅡ）蛋白及AngⅡ 1 型受体（AngiotensinⅡ type1 receptor，AT1R）、AngⅡ 2 型受体（AngⅡ type2 receptor，AT2R）mRNA 表达的影响。结果 与正常对照组相比，LPC 可显著上调HUVECs AngⅡ蛋白及AT1R mRNA 的表达。结论 LPC 能够上调人脐静脉内皮细胞血管紧张素系统部分组分的表达。

关键词: 溶血磷脂酰胆碱, 人脐静脉内皮细胞, 血管紧张素系统

Abstract:

Objective To observe the effect of Lysophosphatidyl choline (LPC) on Renin-angiotensin system (RAS) of human umbilical vein endothelial cells (HUVECs). Methods HUVECs were cultured in vitro. The study was designated to 4 groups: (1) normal control, (2) low-concentration LPC group (10μg/L),(3) middle-concentration LPC group (20μg/L), (4) high-concentration LPC group（40μg/L）.Expression of angiotensinⅡ (AngⅡ ) and AngⅡ type1 receptor(AT1R), AngⅡ type2 receptor(AT2R) mRNA were observed by radio-immunity and RT-PCR, respectively. Results Compared with normal control group, LPC could significantly up- regulate the expression of AngⅡ and AT1R mRNA. Conclusion LPC could partly up-regulate the expression of RSA of human umbilical vein endothelial cells.

Key words: Lysophosphatidyl choline, Human umbilical vein endothelial cells, Renin- angiotensin system（RAS）