热带病与寄生虫学

热带病与寄生虫学 ›› 2014, Vol. 12 ›› Issue (1): 47-49.doi: 10.3969/j.issn.1672-2302.2014.01.016

• 论著 • 上一篇    下一篇

反相高效液相色谱法同时测定食品中的几种重要金属元素

杨林飞,胡卓,方英,钟燕,胡迎春,张建华   

  1. 湖南省株洲市疾病预防控制中心
  • 出版日期:2014-03-10 发布日期:2014-05-12

Effect of reversed phase high performance liquid chromatographic method for the detection of several kinds of important metal elemens in food

Yang Linfei, Hu Zhuo, Fang Ying, Zhong Yan, Hu Yingchun, Zhang Jianhua.   

  1. Zhuzhou City Center for Disease Control and Prevention, Zhuzhou 412000, China
  • Online:2014-03-10 Published:2014-05-12

被引次数

5

摘要： 目的 验证用反相高效液相色谱法同时测定食品中的几种金属元素的效果。方法 用2-2-噻唑偶氮，5-二乙氨基酚为柱前衍生试剂，甲醇（内含0.30%磷酸酸）和pH4.6磷酸—磷酸钠缓冲溶液梯度洗脱为流动相，Waters Nova-Pak-18液相色谱柱为固定相。紫外二极管矩阵检测器检测，反相高效液相色谱法分离测定食品中铁、钴、镍、铜、锌和锰。结果 根据信噪S/N=4.0各种金属离子的检测限分别为：铁4.0μg/L，钴5.0/Lμg，镍4.8 μg/L ，铜3.0μg/L，锌4.8μg/L，锰6.0μg/L，本方法相对标准偏差在2.0%～3.8%之间，标准回收率在95%～108%。结论 本方法可用于食品中痕量铁、钴、镍、铜、锌、锰的测定，结果令人满意。

关键词: 高效液相色谱法, 2-2-噻唑偶氮, 5一二乙氨基酚, 金属元素

Abstract:

Objective To verify the effect of reversed phase high performance liquid chromatographic method for the detection of several kinds of metal elements in food. Methods 2-2 Lhiazole azo, 5-two amino phenol were used as pre column derivative reagent, methanol (conLaining0.30% phosphoric acid ) and pH4.6 phosphoric acid-sodium phosphate buffer solution were used as the mobile phase gradient elution, Waters Nova-Pak-18 liquid chromatography column was used as stationary phase. UV diode array detector and reversed phase high performance liquid chromatographic method were used to separate and detect the iron, cobalt, nickel, copper, zinc and manganese in food. Results According to the letter S / N = 4, the detection limit of the metal ions of iron, cobalt, nickel, copper, zinc and manganese was 4.0 μg/ L, 5.0μg/ L, 4.8μg/ L, 3.0μg/ L, 4.8μg/ L, 6.0μg/ L, respectively. The relative standard deviation was 2.0% ～3.8% ,standard recovery rate was 95%-108%. Conclusion The method can be used to detect the trace of iron,cobalt, nickel, copper, zinc, manganese in food with satisfactory results.

Key words: High performance liquid chromatographic method, 2-2 Lhiazole azo, 5-two amino phenol, Metal element