热带病与寄生虫学

热带病与寄生虫学 ›› 2013, Vol. 11 ›› Issue (2): 91-92,101.doi: 10.3969/j.issn.1672-2302.2013.02.009

• 论著 • 上一篇    下一篇

结肠癌合并血吸虫感染患者癌细胞p53基因的表达

吴平平   

  • 出版日期:2013-02-25 发布日期:2013-06-08

Expression of gene p53 in colorectal cancer cells for patients with schistosomiasis

Wu Pingping   

  1. Department of Pathology, The Second People′s Hospital of Wuhu City, Wuhu 241000, China
  • Online:2013-02-25 Published:2013-06-08

被引次数

2

摘要： 目的 研究原发性结肠癌细胞p53基因mRNA的表达水平及合并血吸虫感染后的差异，探讨其与患者临床病理特征的关系。 方法 38例原发性结肠癌患者分为两组，A组（合并血吸虫感染）20例和B组（未合并血吸虫感染）18例。应用实时荧光定量 PCR和相对定量分析法检测患者肿瘤组织中的p53 mRNA。 结果 p53 mRNA在两组中均可检出，A组中的基因表达水平显著高于B组（P＜0.05）。p53基因mRNA表达水平与年龄、性别相关无显著性，与肿瘤大小、有无淋巴结转移相关具有显著性。结论 p53基因mRNA的高水平表达与结肠癌的侵袭和发展具有显著性差异，血吸虫感染可能对结肠癌患者p53基因的突变有一定影响。

关键词: 肠癌, 血吸虫感染, p53基因, 实时荧光定量PCR

Abstract:

Objective To investigate the association of p53 mRNA expression level with clinic pathological characteristics on patients with colorectal cancer with/without schistosomias. Methods Tissue specimens were obtained from 38 patients undergoing surgery. Real-time quantitative reverse transcriptional PCR (qRT-PCR) was performed for amplification of p53 mRNA from the 20 tumor tissues（A group: colorectal cancer with schistosomias）and 18 tumor tissues (B group: colorectal cancer without schistosomias）. Results P53 mRNA expression was detected in all tissues, and the level of p53 mRNA was significantly higher in group A than in group B. The level of p53 mRNA was highly correlated with tumor size and lymph node metastasis, but not with the patient's sex and age. Conclusion The level of p53 mRNA was correlated to metastasis of colorectal cancer. The schistosome infection may have some influence on expression of gene p53 in colorectal cancer.

Key words: Colorectal cancer, Schistosome infection, Gene p53, qRT-PCR