热带病与寄生虫学

热带病与寄生虫学 ›› 2013, Vol. 11 ›› Issue (2): 89-90.doi: 10.3969/j.issn.1672-2302.2013.02.008

• 论著 • 上一篇    下一篇

美蓝还原酶法应用于突发水污染微生物指标快速检测的研究

邱颖   王敏娣   邱贺民   

  • 出版日期:2013-02-25 发布日期:2013-06-08
  • 基金资助:

    廊坊市科学技术研究与发展计划项目（2012013063）

Study on methylene blue reductive enzymatic application in rapid detection on microorganism for sudden water pollution

Qiu Ying, Wang Mindi, Qiu Hemin   

  1. Langfang Center for Disease Control and Prevention，Langfang 065000, China.
  • Online:2013-02-25 Published:2013-06-08

摘要： 目的 探讨美蓝还原酶法快速检测突发水污染微生物指标即菌落总数的可行性。 方法 通过对美蓝的褪色时间与GB/T5750.12-2006平板计数法的比较，对菌落总数进行检测。 结果 美蓝的还原褪色时间与菌量的多少呈反比，即菌量越大美蓝褪色时间越短。 结论 美蓝还原酶法操作简便，快速，结果准确可靠，可应用于突发水污染的菌落总数的快速检测。

关键词: 美蓝还原酶法, 菌落总数, 快速检测, 突发水污染

Abstract:

Objective To study the feasibility that methylene blue reductase method to the rapid detection on microorganism index in sudden water pollution. Methods The total number of colonies was tested by the comparison between the fading time of methylene blue and the GB/T5750.12-2006 plate count method. Results There was an inverse relationship between the fading time of methylene blue and total number of colonies. The more the number of colonies, the shorter the fading time of methylene blue. Conclusion Methylene blue reduction method is simple, rapid, accurate, reliable and can be applied to rapid detection of the total bacterial colonies in sudden water pollution.

Key words: