新疆阿勒泰地区多房棘球蚴人体分离株nad2基因多态性分析

doi:10.20199/j.issn.1672-2302.2025.04.010

热带病与寄生虫学 ›› 2025, Vol. 23 ›› Issue (4): 245-248.doi: 10.20199/j.issn.1672-2302.2025.04.010

新疆阿勒泰地区多房棘球蚴人体分离株nad2基因多态性分析

赵江山¹(), 郭宝平², 史光忠¹, 亚里昆·买买提依明¹, 阿迪力·司马义¹, 王蒴¹()

1.新疆维吾尔自治区疾病预防控制中心，新疆乌鲁木齐 830002
2.新疆医科大学第一附属医院

收稿日期:2025-04-01 出版日期:2025-08-20 发布日期:2025-09-19
通信作者: 王蒴，E-mail: 490467434@qq.com
作者简介:赵江山，男，硕士，高级实验师，研究方向：寄生虫病与布病预防控制。E-mail: zjscdc@163.com
基金资助:
新疆维吾尔自治区重点研发项目(2022B03013-1)

Polymorphism analysis of nad2 gene of Echinococcus multilocularis larvae isolates from human in Altay Prefecture of Xinjiang

ZHAO Jiangshan¹(), GUO Baoping², SHI Guangzhong¹, YALIKUN Maimaitiyiming¹, ADILI Simayi¹, WANG Shuo¹()

1. Center for Disease Control and Prevention of Xinjiang Uygur Autonomous Region, Urumqi 830002, Xinjiang Uygur Autonomous Region, China
2. The First Affiliated Hospital of Xinjiang Medical University

Received:2025-04-01 Online:2025-08-20 Published:2025-09-19
Contact: WANG Shuo, E-mail: 490467434@qq.com

摘要/Abstract

摘要：

目的了解新疆阿勒泰地区多房棘球蚴人体分离株的基因型及遗传多样性，为当地多房棘球蚴病的防控提供理论依据。方法收集2021—2024年新疆医科大学第一附属医院收治的阿勒泰地区多房棘球蚴病患者手术切除的肝脏病灶组织，提取组织DNA，采用PCR法对nad2基因进行扩增、测序。将测序成功的基因序列上传NCBI网站进行Blast比对，运用DnaSP 5软件进行nad2基因多态性分析，应用NetWork 10绘制单倍型网络图，利用MEGA 11软件中的neighbor-joining法建立nad2基因系统进化树。结果共获得36份肝脏病灶组织，经PCR扩增，36份样本均在882 bp处出现特异性条带，符合预期大小。基因多态性结果显示，nad2基因共有3种单倍型，属于亚洲群，单倍型多样性为0.341，核苷酸多样性为0.000 76，Hap1为主要单倍型（29/36，占80.56%）。系统进化树显示，分离株与中国新疆新源县（KT965464）、哈萨克斯坦（AB461406）、波兰（KY205700）、加拿大（MT429276）、日本北海道（AB461407）和中国四川省（AB461408）等地多房棘球绦虫参考序列具有较近亲缘关系。结论阿勒泰地区人体感染多房棘球绦虫属于亚洲群，遗传上较为保守，优势单倍型为Hap1。

关键词: 多房棘球蚴, nad2基因, 基因多态性, 新疆阿勒泰地区

Abstract:

Objective To understand the genotype and genetic diversity of Echinococcus multilocularis larvae isolates from human in Altay Prefecture of Xinjiang, and to provide a theoretical basis for the prevention and control of alveolar echinococcosis (AE). Methods The liver lesion tissues were obtained from patients with AE in Altay Prefecture of Xinjiang who were treated in the First Affiliated Hospital of Xinjiang Medical University between 2021 and 2024, and from which the tissue DNA was extracted. The nad2 gene was amplified and sequenced by PCR. The successfully sequenced gene sequence was uploaded to the NCBI website for Blast comparison, the nad2 gene polymorphism was analyzed using DnaSP 5 software, the haplotype network diagram was drawn by NetWork 10, and the nad2 gene phylogenetic tree was established by using the neighbor-joining method in MEGA 11 software. Results A total of 36 liver lesion tissues were obtained. After PCR amplification, specific bands at 882 bp were observed in all 36 samples, which was in line with the expected size. The results of gene polymorphism showed that there were three haplotypes in nad2 gene belong to the Asian clade, with a haplotype diversity of 0.341 and nucleotide diversity of 0.000 76. The Hap1 was the main haplotype (29/36, 80.56%). The systematic evolution tree showed that the isolates have a close kinship with the reference sequences of Echinococcus multilocularis from Xinyuan County of Xinjiang (KT965464), Kazakhstan (AB461406), Poland (KY20700), Canada (MT429276), Hokkaido of Japan (AB461407) and Sichuan (AB461408). Conclusion Human Echinococcus multilocularis isolated from subjects in Altay Prefecture of Xinjiang belongs to Asian clade, and is genetically relatively conserved. The dominant haplotype of it is Hap1.

Key words: Echinococcus multilocularis larvae, Nad2 gene, Gene polymorphism, Altay Prefecture of Xinjiang

中图分类号:

R183.5

赵江山, 郭宝平, 史光忠, 亚里昆·买买提依明, 阿迪力·司马义, 王蒴. 新疆阿勒泰地区多房棘球蚴人体分离株nad2基因多态性分析[J]. 热带病与寄生虫学, 2025, 23(4): 245-248.

ZHAO Jiangshan, GUO Baoping, SHI Guangzhong, YALIKUN Maimaitiyiming, ADILI Simayi, WANG Shuo. Polymorphism analysis of nad2 gene of Echinococcus multilocularis larvae isolates from human in Altay Prefecture of Xinjiang[J]. Journal of Tropical Diseases and Parasitology, 2025, 23(4): 245-248.

图/表 3

图1

图2

图3

参考文献 22

[1]	徐凯, 王海久, 张丽, 等. 多房棘球蚴对宿主肝细胞损害机制的研究进展[J]. 中国寄生虫学与寄生虫病杂志, 2021, 39(2):256-260. doi: 10.12140/j.issn.1000-7423.2021.02.020
[2]	Knapp J, Gottstein B, Saarma U, et al. Taxonomy, phylogeny and molecular epidemiology of Echinococcus multilocularis: From fundamental knowledge to health ecology[J]. Vet Parasitol, 2015, 213(3/4):85-91.
[3]	Umhang G, Bastid V, Avcioglu H, et al. Unravelling the genetic diversity and relatedness of Echinococcus multilocularis isolates in Eurasia using the EmsB microsatellite nuclear marker[J]. Infect Genet Evol, 2021,92:104863.
[4]	马增光. 新疆昭苏盆地野生宿主多房棘球蚴病的流行病学调查[D]. 乌鲁木齐: 新疆农业大学, 2014.
[5]	Guo BP, Zhang ZZ, Guo YZ, et al. High endemicity of alveolar echinococcosis in Yili Prefecture,Xinjiang Autonomous Region,the People’s Republic of China: Infection status in different ethnic communities and in small mammals[J]. PLoS Negl Trop Dis, 2021, 15(1):e0008891.
[6]	Nakao M, Xiao N, Okamoto M, et al. Geographic pattern of genetic variation in the fox tapeworm Echinococcus multilocularis[J]. Parasitol Int, 2009, 58(4):384-389.
[7]	Vanhove MPM, Briscoe AG, Jorissen MWP, et al. The first next-generation sequencing approach to the mitochondrial phylogeny of African monogenean parasites (Platyhelminthes: Gyrodactylidae And Dactylogyridae)[J]. BMC Genomics, 2018, 19(1):520. doi: 10.1186/s12864-018-4893-5 pmid: 29973152
[8]	Clement M, Posada D, Crandall KA. TCS a computer program to estimate gene genealogies[J]. Mol Ecol, 2000, 9(10):1657-1659. doi: 10.1046/j.1365-294x.2000.01020.x pmid: 11050560
[9]	Guo BP, Zhang ZZ, Zheng XT, et al. Prevalence and molecular characterization of Echinococcus granulosus sensu stricto in northern Xinjiang, China[J]. Korean J Parasitol, 2019, 57(2):153-159.
[10]	Rozas J, Ferrer-Mata A, Sánchez-DelBarrio JC, et al. DnaSP 6: DNA sequence polymorphism analysis of large data sets[J]. Mol Biol Evol, 2017, 34(12):3299-3302. doi: 10.1093/molbev/msx248 pmid: 29029172
[11]	魏玉环, 刘华, 李武军, 等. 西藏阿里地区细粒棘球蚴人体分离株nad1基因多态性分析[J]. 中国寄生虫学与寄生虫病杂志, 2020, 38(1):17-21,29. doi: 10.12140/j.issn.1000-7423.2020.01.003
[12]	Uakhit R, Smagulova A, Syzdykova A, et al. Genetic diversity of Echinococcus spp. in wild carnivorous animals in Kazakhstan[J]. Vet World, 2022, 15(6):1489-1496.
[13]	尚婧晔, 张光葭, 喻文杰, 等. 多房棘球绦虫遗传多态性研究进展[J]. 中国寄生虫学与寄生虫病杂志, 2020, 38(5):637-641,646. doi: 10.12140/j.issn.1000-7423.2020.05.017
[14]	郭永忠, 初伊明, 阿尔新·卡德尔, 等. 新疆伊犁地区尼勒克县人群泡球蚴感染病例调查及nad2、cob基因片段分析[J]. 中国人兽共患病学报, 2017, 33(12):1066-1070. doi: 10.3969/j.issn.1002-2694.2017.12.003
[15]	尚婧晔, 张光葭, 喻文杰, 等. 中国西北部地区多房棘球绦虫cox1基因多态性研究[J]. 中国病原生物学杂志, 2021, 16(2):137-142.
[16]	Alvarez Rojas CA, Kronenberg PA, Aitbaev S, et al. Genetic diversity of Echinococcus multilocularis and Echinococcus granulosus sensu lato in Kyrgyzstan: The A2 haplotype of E. multilocularis is the predominant variant infecting humans[J]. PLoS Negl Trop Dis, 2020, 14(5):e0008242.
[17]	Bohard L, Lallemand S, Borne R, et al. Complete mitochondrial exploration of Echinococcus multilocularis from French alveolar echinococcosis patients[J]. Int J Parasitol, 2023, 53(10):555-564. doi: 10.1016/j.ijpara.2023.03.006 pmid: 37148987
[18]	Guo BP, Cairen, Wu JY, et al. The A2 haplotype of Echinococcus multilocularis is the predominant variant infecting humans and dogs in Yili Prefecture, Xinjiang[J]. Infect Genet Evol, 2024,119:105581.
[19]	赵江山, 王蒴, 张月, 等. 新疆南疆地区小型啮齿动物多房棘球蚴感染现状与基因型[J]. 中国热带医学, 2025, 25(1):46-51. doi: 10.13604/j.cnki.46-1064/r.2025.01.08
[20]	吴川川. 中国西部地区多房棘球绦虫mtDNA基因多态性分析[D]. 乌鲁木齐: 新疆农业大学, 2016.
[21]	王旭, 候岩岩, 王莹, 等. 新疆新源县和四川石渠县啮齿动物及家畜棘球绦虫线粒体cox1基因遗传多态性分析[J]. 中国寄生虫学与寄生虫病杂志, 2020, 38(1):22-29. doi: 10.12140/j.issn.1000-7423.2020.01.004
[22]	Wu CC, Zhang WB, Ran B, et al. Genetic variation of mitochondrial genes among Echinococcus multilocularis isolates collected in western China[J]. Parasit Vectors, 2017, 10(1):265.

新疆阿勒泰地区多房棘球蚴人体分离株nad2基因多态性分析

Polymorphism analysis of nad2 gene of Echinococcus multilocularis larvae isolates from human in Altay Prefecture of Xinjiang

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 3

参考文献 22

相关文章 1

编辑推荐

Metrics

本文评价