热带病与寄生虫学

热带病与寄生虫学 ›› 2022, Vol. 20 ›› Issue (2): 94-95,102.

• 临床研究 • 上一篇    下一篇

全自动快速微生物质谱检测系统在流感嗜血杆菌快速鉴定中的价值分析

秦淑国，许元元，赵萍萍，李慧慧，李振鹏   

  1. 皖北煤电集团总医院检验科，安徽 宿州 234001
  • 收稿日期:2021-12-09 出版日期:2022-04-20 发布日期:2022-04-29
  • 作者简介:秦淑国，男，本科，主任技师，研究方向：微生物及分子生物学。E-mail：QSGXR@163.com
  • 基金资助:
    宿州市科技局课题（SZSKJJZC2021042）

Analysis on the value of automatic rapid microbial mass spectrometry detection system in rapid identification of Haemophilus influenzae

QIN Shu-guo, XU Yuan-yuan, ZHAO Ping-ping, LI Hui-hui, LI Zhen-peng   

  1. Department of Clinical Laboratory, General Hospital of Wanbei Coal-Electricity Group, Suzhou 234001, Anhui Province, China
  • Received:2021-12-09 Online:2022-04-20 Published:2022-04-29

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摘要： 目的 分析全自动快速微生物质谱检测系统（MALDI-TOF）在鉴定流感嗜血杆菌（Haemophilus influenzae, Hi）中的应用价值。方法 收集皖北煤电集团总医院2020年7月至2021年3月间住院患者1 135份痰液样本，提取来自不同患者的Hi 30株，分别采用MALDI-TOF、全自动细菌鉴定仪（Vitek）及M-H琼脂药敏试验对所有受检标本进行细菌种属鉴定，记录并比较3种方法对Hi菌株的鉴定结果。结果 MALDI-TOF对Hi菌株的检出率为96.67%，高于Vitek的86.67%，但差异无统计学意义（χ2=1.333，P=0.248）；也高于M-H琼脂试验的73.33%，差异有统计学意义（χ2=5.143，P=0.023）。3种方法检测用时分别为（0.15±0.01） h、（17.04±5.93） h和（36.78±9.58） h，差异有统计学意义（F=18.258，P<0.001）；进一步用LSD-t检验对3种方法用时进行两两比较，差异均有统计学意义（P均<0.001）。结论 全自动快速微生物质谱检测系统可对Hi进行有效鉴定，具有成本低、准确率高、操作简便、检测用时短等明显优点，在临床Hi的鉴定中具有较高应用价值。

关键词: 全自动快速微生物质谱检测系统, 流感嗜血杆菌, 鉴定

Abstract: Objective To analyze the application value of automatic rapid microbial mass spectrometry detection system (MALDI-TOF) in the identification of Haemophilus influenzae (Hi). Methods A total of 1 135 aliquots of sputum samples were obtained from inpatients treated in our hospital between July 2020 and March 2021, 30 strains of Hi were extracted from different patients and characterized for the bacterial species using MALDI-TOF, automatic bacterial identification instrument (Vitek) and M-H agar drug susceptibility test, respectively. The results of the three methods in identifying Hi strains were recorded and compared. Results The detection rate of Hi strains was higher by MALDI-TOF than by Vitek (96.67% vs. 86.67%). The difference was insignificant (χ2=1.333, P=0.248), yet significant compared to M-H agar test (73.33%; χ2=5.143, P=0.023). Time consumption was the shortest by MALDI-TOF test (0.15±0.01) h, followed by Vitek method (17.04±5.93) h, yet M-H agar test consumed the longest time (36.78±9.58) h. The difference was significant among the three tests (F=18.258, P<0.001). Further paired comparison of the time consumption by three methods using LSD-t test also indicated statistical difference (all P<0.001). Conclusion Automatic rapid microbial mass spectrometry detection system can be higher clinical value in effectively identifying Hi, and obviously has advantages such as low cost, high accuracy, simple operation, automatic display and interpretation of the results as well as shorter detection time.

Key words: MALDI-TOF, Haemophilus influenzae, Identification

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