热带病与寄生虫学

热带病与寄生虫学 ›› 2021, Vol. 19 ›› Issue (5): 264-.

• 实验研究 • 上一篇    下一篇

温度胁迫对湖北钉螺部分呼吸酶的影响

潘中武1,桂琳1,胡霞2,章琴3,周书林3
  

  1. 1. 皖南医学院医学微生物与免疫学教研室,安徽 芜湖 241002; 2. 安徽中医药高等专科学校解剖学教研室; 3. 皖南医学院医学寄生虫学教研室
  • 出版日期:2021-10-20 发布日期:2021-11-03
  • 通信作者: 周书林,E-mail: 625950747@ qq. com
  • 作者简介:潘中武,男,硕士,讲师,研究方向:血吸虫病控制。 E-mail:719653530@ qq. com
  • 基金资助:
    安徽省自然科学基金项目(11040606M214);安徽省教育厅高校自然科学研究重点项目(KJ2017A251)

Effects of temperature stress on some respiratory enzymes in Oncomelania hupensis 

PAN Zhong-wu1,GUI Lin1,HU Xia2,ZHANG Qin3,ZHOU Shu-lin3   

  1. 1. Department of Microbiology and Immunology,Wannan Medical College,Wuhu 241002,Anhui Province,China; 2. Department of Human Anatomy,Anhui College of Traditional Chinese Medicine; 3. Department of Medical Parasitology,Wannan Medical College
  • Online:2021-10-20 Published:2021-11-03

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摘要： 目的 探索温度胁迫(高温和低温)情况下湖北钉螺体内细胞色素氧化酶和琥珀酸脱氢酶两种酶的含量变化情况。 方法 将钉螺随机分成对照组、低温组和高温组,分别放置于 20 ℃ 、4 ℃和 37 ℃环境中,24 h 后取钉螺软体组织,测定各组钉螺细胞色素氧化酶和琥珀酸脱氢酶的含量。 结果 对照组、低温组和高温组三组钉螺软体组织中细胞色素氧化酶含量分别为(27. 11±2. 31) U / L、(21. 90±0. 32)U / L 和(21. 42±1. 84)U / L,三组间不全相同,且差异有统计学意义( F= 10. 145,P<0. 05);琥珀酸脱氢酶含量分别为(1. 30±0. 002) U / L、(1. 24±0. 05) U / L 和(1. 12±0. 02)U / L,三组间不全相同,且差异有统计学意义( F= 124. 624,P<0. 05)。 结论 湖北钉螺在温度胁迫状态下,其软体组织中细胞色素氧化酶和琥珀酸脱氢酶的含量均降低。

关键词: 湖北钉螺, 温度胁迫, 细胞色素氧化酶, 琥珀酸脱氢酶

Abstract: Objective To investigate the changes of cytochrome oxidase(COX) and succinate dehydrogenase ( SDH) contents in Oncomelania hupensis(O. hupensis) under laboratory condition and temperature stress ( high temperature and low temperature). Methods O. hupensis snails were randomly divided into control group ( T = 20 ℃ ),low temperature group (T = 4 ℃ ) and high temperature group (T = 37 ℃ ). Soft tissues of the snails were taken at 24 h after treatment by the different temperature to measure the changes of COX and SDH level in each group. Results The content of COX and SDH in the soft tissues was (27. 11±2. 31) U / L,(21. 90± 0. 32) U / L and ( 21. 42± 1. 84) U / L;( 1. 30± 0. 002) U / L,( 1. 24±0. 05)U / L and (1. 12 ± 0. 02) U / L,respectively for the control,low temperature group and high temperature group. The difference was significant among groups( F= 10. 145,P<0. 05 for COX; F= 124. 624,P<0. 05 for SDH). Conclusion COX and SDH contents tend to decrease in the soft tissue of O. hupensis under temperature stress.

Key words: Oncomelania hupensis, Temperature stress, Cytochrome oxidase, Succinate dehydrogenase

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